Western Blot (WB)
(PMF1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PMF1 antibody detected the PMF1 protein (arrow).)
Rabbit anti-Human PMF1 Polyclonal Antibody | anti-PMF1 antibody
PMF1, ID (PMF1, Polyamine-modulated factor 1) (Biotin)
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PMF1; Polyclonal Antibody; ID (PMF1; Polyamine-modulated factor 1) (Biotin); Polyamine-modulated factor 1; anti-PMF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
205
Applicable Applications for anti-PMF1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PMF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-135 amino acids from the Central region of human PMF1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PMF1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PMF1 antibody detected the PMF1 protein (arrow).)
Western Blot (WB)
(PMF1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PMF1 antibody detected the PMF1 protein (arrow).)
Related Product Information for anti-PMF1 antibody
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Product Categories/Family for anti-PMF1 antibody
References
Aleman, A., et al. Clin. Cancer Res. 14(24):8236-8243(2008). Kline, S.L., et al. J. Cell Biol. 173(1):9-17(2006). Obuse, C., et al. Nat. Cell Biol. 6(11):1135-1141(2004). Wang, Y., et al. Biochem. J. 366 (PT 1), 79-86 (2002): Wang, Y., et al. Biochem. J. 355 (PT 1), 45-49 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
polyamine-modulated factor 1 isoform 2
NCBI Official Synonym Full Names
polyamine modulated factor 1
NCBI Official Symbol
PMF1
NCBI Protein Information
polyamine-modulated factor 1
UniProt Protein Name
Polyamine-modulated factor 1
Protein Family
UniProt Gene Name
PMF1
UniProt Synonym Gene Names
PMF-1
Uniprot Description
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Research Articles on PMF1
Similar Products
Product Notes
The PMF1 pmf1 (Catalog #AAA6335160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PMF1, ID (PMF1, Polyamine-modulated factor 1) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PMF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PMF1 pmf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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