Rabbit PLGF Polyclonal Antibody | anti-PLGF antibody

RABBIT ANTI HUMAN PLGF

Cat. Num. AAA222860

• Gene Names: PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: PLGF; Polyclonal Antibody; RABBIT ANTI HUMAN PLGF; anti-PLGF antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 149

• Applicable Applications for anti-PLGF antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: This purified human PLGF antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human PLGF antibody as the detection reagent and recombinant human PLGF as the standard.; Western Blot: This purified human PLGF antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human PLGF as the positive control.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml; Immunohistology - Paraffin: Maximum Dilution: 2.0ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human PlGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human PLGF (MBS222860) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Testing Data #2

(Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human PLGF (MBS222860) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-PLGF antibody

Rabbit anti human PlGF antibody recognizes human placental growth factor (PlGF). PlGF is a dimeric glycoprotein and member of the vascular endothelial growth factor (VEGF) family, involved in angiogenesis, and the stimulation of endothelial cell proliferation and migration. The three isoforms PlGF-1, PlGF-2 and PlGF-3 are present in most placental tissues and act through binding to the VEGFR-1/FLT1 receptor. The involvement of PlGF in angiogenesis has made PlGF a focus area in the study of inflammatory diseases such as rheumatoid arthritis and psoriasis, and PlGF is used as a biochemical marker in cardiovascular disease, and also detected at reduced levels during preeclampsia (Krauss et al. 2004).

NCBI and Uniprot Product Information

• NCBI GI #: 333033785
• NCBI GeneID: 5228
• NCBI Accession #: NP_001193941.1
• NCBI GenBank Nucleotide #: NM_001207012.1
• UniProt Accession #: P49763; Q07101; Q9BV78; Q9Y6S8
• Molecular Weight: 19,338 Da
• NCBI Official Full Name: placenta growth factor isoform 2
• NCBI Official Synonym Full Names: placental growth factor
• NCBI Official Symbol: PGF
• NCBI Official Synonym Symbols: PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• NCBI Protein Information: placenta growth factor; placental growth factor, vascular endothelial growth factor-related protein
• UniProt Protein Name: Placenta growth factor
• UniProt Gene Name: PGF
• UniProt Synonym Gene Names: PGFL; PLGF; PlGF
• UniProt Entry Name: PLGF_HUMAN

NCBI Description

This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity

Biological Process: response to drug; organ regeneration; female pregnancy; signal transduction; cellular response to hormone stimulus; positive regulation of angiogenesis; cell-cell signaling; ureteric bud branching; positive regulation of cell division; positive regulation of cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell differentiation