Rat CD134 Monoclonal Antibody | anti-CD134 antibody

CD134, Mouse (OX40)

Cat. Num. AAA4381531

• Reactivity: Mouse; Does not react with Human.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD134; Monoclonal Antibody; Mouse (OX40); IMD16; Lymphoid activation antigen (ACT35); OX40 cell surface antigen; OX40L receptor; TAX transcriptionally activated glycoprotein 1 receptor; Tumor necrosis factor receptor superfamily member 4 (TNFRSF4); Txgp1l; anti-CD134 antibody

• Host: Rat
• Reactivity: Mouse; Does not react with Human.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: OX-86
• Specificity: CD134 is a type I integral membrane protein. This receptor is expressed on activated CD4+and CD8+T cells and B cells. The CD134 binds to CD134 ligand (CD252) to provide a costimulatory signal that is independent of CD28. CD134 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. CD134 is also involved in the stimulation of T cells, T dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. CD134 is expressed on activated CD4+ T lymphocytes, and its ligand, CD134L, is found preferentially on activated B cells. Engagement of CD134 with its ligand, CD134L, delivers a strong costimulatory signal to effector T cells.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 272

• Applicable Applications for anti-CD134 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Does not block binding of OX40L.; FC/FACS: 0.5-1ug/million cells in 0.1ml; IF: 0.5-1ug/ml
• Positive Control: MPBMC's. Spleen, Thymus or Lymph Node.
• Immunogen: Purified recombinant Mouse OX-40 antigen
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 4 E2; 4 87.68 cM (Mouse)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified CD134 Rat Monoclonal Antibody (OX-86). Confirmation of Integrity and Purity of Antibody.)

References

1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Agonist). 2. del Rio ML, et al. 2011. Transpl. Int. 24:501. (Flow). 3. Higgins LM, et al. 1999. J. Immunol. 162:486. (Flow IHC)

NCBI and Uniprot Product Information

• NCBI GI #: 1352652
• NCBI GeneID: 22163
• UniProt Accession #: P47741
• Molecular Weight: 50kDa
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 4
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 4
• UniProt Gene Name: Tnfrsf4
• UniProt Synonym Gene Names: Ox40; Txgp1
• UniProt Entry Name: TNR4_MOUSE

Uniprot Description

TNFRSF4: Receptor for TNFSF4/OX40L/GP34.

Protein type: Apoptosis; Receptor, misc.; Membrane protein, integral

Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity

Biological Process: regulation of apoptosis; T cell proliferation; tumor necrosis factor-mediated signaling pathway; negative regulation of transcription factor activity; negative regulation of cytokine secretion; programmed cell death; positive regulation of B cell proliferation; cellular defense response; positive regulation of immunoglobulin secretion; negative regulation of transcription, DNA-dependent; regulation of protein kinase activity; inflammatory response