Rat anti-Mouse CD24/HSA Monoclonal Antibody

Rat Anti-Mouse CD24/HSA-FITC

Cat. Num. AAA673272

• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD24/HSA; Monoclonal Antibody; Rat Anti-Mouse CD24/HSA-FITC; Rat Anti-Mouse CD24/HSA - FITC; Rat Anti-Mouse CD24/HSA; anti-CD24/HSA antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone Number: 91
• Specificity: Mouse heat stable antigen (HSA); Mr 35^-45 kDa; CD24, also known as heat stable antigen, is a phosphatidylinositol^-linked cell surface glycoprotein that is expressed at all stages of B cell development and on most thymocytes.^1^-3 The absence of expression from mature T cells is closely associated with their maturation from CD4^+CD8^+CD24^+ thymocytes to either CD4^+CD8^-CD24^- or CD4^-CD8^+CD24^- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. ^4^-6 Its ligand is P^-selectin (CD62P). ^7 CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. ^1^-3 The monoclonal antibody 91 can be used to inhibit B^-cell differentiation. ^8
• Form/Format: Fluorescein (FITC) Conjugate

• Applicable Applications for anti-CD24/HSA antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Flow Cytometry Suggested Dilution: <= 4 ug/10^6 cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this data sheet.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. Bruce, J., F.W. Symington, T.J. McKearn, and J. Sprent. 1981. J. Immunol. 127:2496.
2. Alterman, L.A., I.N. Crispe, and C. Kinnon. 1990. Eur. J. Immunol. 20:1597.
3. Davidson, W.F., T.M. Chused, and H.C. Morse. 1981. Immunogenetics 13:421.
4. Rougon, G., L.A. Alterman, K. Dennis, X.J. Guo, and C. Kinnon. 1991. Eur. J. Immunol. 21:1397.
5. Wilson, A., L.M. Day, R. Scollay, and K. Shortman. 1984. Immnol. Rev. 82:79.
6. Shortman, K., A. Wilson, M. Egerton, M. Pearse, and R. Scollay. 1988. Cell. Immunol. 117:312.
7. Aigner, S., M. Ruppert, M. Hubbe, M. Sammar, Z. Sthoeger, E. C. Butcher, D. Vestweber, and P. Altevogt. 1995. International Immunology 7:1557.
8. Kearney, J.F. Personal communication.