Western Blot (WB)
(PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).)
Rabbit anti-Human PLCL2 Polyclonal Antibody | anti-Plcl2 antibody
PLCL2, CT (PLCL2, KIAA1092, PLCE2, Inactive phospholipase C-like protein 2, Phospholipase C-epsilon-2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50
Western Blot (WB)
(PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).)
Western Blot (WB)
(PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).)
Immunohistochemistry (IHC)
(PLCL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PLCL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PLCL2 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PLCL2 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(PLCL2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PLCL2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
PLCL2: May play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Phospholipase
Cellular Component: cytoplasm
Molecular Function: signal transducer activity; phosphoric diester hydrolase activity; GABA receptor binding; phosphoinositide phospholipase C activity
Biological Process: negative regulation of B cell receptor signaling pathway; regulation of peptidyl-serine phosphorylation; B-1a B cell differentiation; lipid metabolic process; signal transduction; regulation of synaptic transmission, GABAergic; gamma-aminobutyric acid signaling pathway; B cell proliferation during immune response
Research Articles on Plcl2
Similar Products
Product Notes
The Plcl2 plcl2 (Catalog #AAA6005631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLCL2, CT (PLCL2, KIAA1092, PLCE2, Inactive phospholipase C-like protein 2, Phospholipase C-epsilon-2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLCL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Plcl2 plcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
