Rabbit anti-Human, Mouse PLB Polyclonal Antibody | anti-PLB antibody

PLB-T17 (PLN, PLB, Cardiac phospholamban) (MaxLight 490)

Cat. Num. AAA6334647

• Gene Names: PLN; PLB; CMD1P; CMH18
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PLB; Polyclonal Antibody; PLB-T17 (PLN; Cardiac phospholamban) (MaxLight 490); PLN; Cardiac phospholamban; anti-PLB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLB-T17. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-PLB antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 of human PLB-T17.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue usingMBS648568, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648568 for immunohistochemistry.)

Product Categories/Family for anti-PLB antibody

Antibodies; Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4505887
• NCBI GeneID: 5350
• NCBI Accession #: NP_002658.1
• NCBI GenBank Nucleotide #: NM_002667.3
• UniProt Accession #: P26678
• Molecular Weight: 6,109 Da
• NCBI Official Full Name: cardiac phospholamban
• NCBI Official Synonym Full Names: phospholamban
• NCBI Official Symbol: PLN
• NCBI Official Synonym Symbols: PLB; CMD1P; CMH18
• NCBI Protein Information: cardiac phospholamban
• UniProt Protein Name: Cardiac phospholamban
• Protein Family: Lysophospholipase
• UniProt Gene Name: PLN
• UniProt Synonym Gene Names: PLB; PLB
• UniProt Entry Name: PPLA_HUMAN

NCBI Description

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]

Uniprot Description

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Membrane protein, integral; Inhibitor

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: sarcoplasmic reticulum membrane; mitochondrion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; vesicle

Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding

Biological Process: cardiac muscle development; negative regulation of calcium ion transport; negative regulation of heart rate; cytosolic calcium ion homeostasis; blood circulation; regulation of calcium ion transport; response to testosterone stimulus; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; regulation of the force of heart contraction; protein homooligomerization

Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18