Immunoblot (IB)
(Immunoblot Analysis: A 0.50μg/mL dilution of this lot detected phospho-PDGFRα (Tyr742) in RIPA lysate of PDGF treated NIH3T3 cells. Immunoblot Analysis Lysates from untreated (lane 1) and PDGF treated (lanes 2 and 3) NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho- PDGFRα (Tyr742). Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho- PDGFRα (Tyr742). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-PDGFRα.)
Rabbit anti-Human, Mouse Platelet Derived Growth Factor Receptor alpha Polyclonal Antibody
Platelet Derived Growth Factor Receptor alpha, phosphorylated (Tyr742) (PDGFRa)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Platelet Derived Growth Factor Receptor alpha; Polyclonal Antibody; phosphorylated (Tyr742) (PDGFRa); Anti -Platelet Derived Growth Factor Receptor alpha; anti-Platelet Derived Growth Factor Receptor alpha antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes phospho-PDGFR (Tyr742), Mr 170-185. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide. Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Applicable Applications for anti-Platelet Derived Growth Factor Receptor alpha antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Peptide corresponding to amino acid region encompassing the human phospho-PDGFRalpha (Tyr742).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunoblot (IB)
(Immunoblot Analysis: A 0.50μg/mL dilution of this lot detected phospho-PDGFRα (Tyr742) in RIPA lysate of PDGF treated NIH3T3 cells. Immunoblot Analysis Lysates from untreated (lane 1) and PDGF treated (lanes 2 and 3) NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho- PDGFRα (Tyr742). Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho- PDGFRα (Tyr742). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-PDGFRα.)
Immunoblot (IB)
(Immunoblot Analysis: A 0.50μg/mL dilution of this lot detected phospho-PDGFRα (Tyr742) in RIPA lysate of PDGF treated NIH3T3 cells. Immunoblot Analysis Lysates from untreated (lane 1) and PDGF treated (lanes 2 and 3) NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho- PDGFRα (Tyr742). Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho- PDGFRα (Tyr742). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-PDGFRα.)
Product Categories/Family for anti-Platelet Derived Growth Factor Receptor alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
platelet derived growth factor receptor alpha
Similar Products
Product Notes
The Platelet Derived Growth Factor Receptor alpha (Catalog #AAA628896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Platelet Derived Growth Factor Receptor alpha, phosphorylated (Tyr742) (PDGFRa) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Platelet Derived Growth Factor Receptor alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the Platelet Derived Growth Factor Receptor alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Platelet Derived Growth Factor Receptor alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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