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ELISA (EIA) (ELISA: Direct Elisa Test. Protein as test antigen. MBS612738 as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of antigen.)

Chicken Platelet Activating Factor Acetylhydrolase, Isoform Ib, beta 30kD Polyclonal Antibody | anti-PAFAH1B2 antibody

Platelet Activating Factor Acetylhydrolase, Isoform Ib, beta 30kD (PAFAH1B2)

Gene Names
PAFAH1B2; PAFAH1B2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by affinity chromatography.
Synonyms
Platelet Activating Factor Acetylhydrolase; Isoform Ib; beta 30kD; Polyclonal Antibody; beta 30kD (PAFAH1B2); Anti -Platelet Activating Factor Acetylhydrolase; anti-PAFAH1B2 antibody
Ordering
For Research Use Only!
Host
Chicken
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes human Platelet Activating Factor Acetylhydrolase, Isoform Ib, beta 30kD. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-PAFAH1B2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use ELISA and Western Blot.
Dilution: Western Blot: 1:500
Immunogen
Recombinant human protein Platelet-activating factor acerylhydrolase, isoform b, beta subunit (aa1-229).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EIA)

(ELISA: Direct Elisa Test. Protein as test antigen. MBS612738 as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of antigen.)

ELISA (EIA) (ELISA: Direct Elisa Test. Protein as test antigen. MBS612738 as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of antigen.)

Western Blot (WB)

(Western Blot: E coli-derived fusion protein as test antigen. MBS612738 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Western Blot (WB) (Western Blot: E coli-derived fusion protein as test antigen. MBS612738 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)
Related Product Information for anti-PAFAH1B2 antibody
Cytosolic PAFAH IB is formed of three subunits, alpha (45kD), beta (30kD, and gamma (29kD). The catalytic activity of PAFAH IB resides in the beta and gamma subunits while the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity. Specifically, PAFAH subunit gamma inactivates platelet-activating factor by removing the acetyl group at the sn-2 position. It plays an important role during the development of the brain. In the adult, it is expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in the brain.
Product Categories/Family for anti-PAFAH1B2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,569 Da
NCBI Official Full Name
platelet-activating factor acetylhydrolase IB subunit beta isoform a
NCBI Official Synonym Full Names
platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)
NCBI Official Symbol
PAFAH1B2
NCBI Official Synonym Symbols
PAFAH1B2
NCBI Protein Information
platelet-activating factor acetylhydrolase IB subunit beta; OTTHUMP00000234977; OTTHUMP00000234979; OTTHUMP00000234980; OTTHUMP00000234981; PAFAH subunit beta; PAF-AH subunit beta; PAF-AH 30 kDa subunit; PAF-AH1b alpha 2 subunit; PAF acetylhydrolase 30 kDa subunit; intracellular platelet-activating factor acetylhydrolase alpha 2 subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa)
UniProt Protein Name
Platelet-activating factor acetylhydrolase IB subunit beta
UniProt Gene Name
PAFAH1B2
UniProt Synonym Gene Names
PAFAHB
UniProt Entry Name
PA1B2_HUMAN

NCBI Description

Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [supplied by RefSeq]

Uniprot Description

PAFAH1B2: Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Lipid Metabolism - ether lipid; Acetyltransferase; EC 3.1.1.47; Lipase

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: cytoplasm; plasma membrane; nucleolus; cytosol

Molecular Function: protein homodimerization activity; platelet-activating factor acetyltransferase activity; protein heterodimerization activity; 1-alkyl-2-acetylglycerophosphocholine esterase activity

Biological Process: spermatogenesis; brain development; lipid metabolic process; positive regulation of macroautophagy; lipid catabolic process

Research Articles on PAFAH1B2

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Product Notes

The PAFAH1B2 pafah1b2 (Catalog #AAA612738) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Platelet Activating Factor Acetylhydrolase, Isoform Ib, beta 30kD (PAFAH1B2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Platelet Activating Factor Acetylhydrolase, Isoform Ib, beta 30kD can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use ELISA and Western Blot. Dilution: Western Blot: 1:500. Researchers should empirically determine the suitability of the PAFAH1B2 pafah1b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Platelet Activating Factor Acetylhydrolase, Isoform Ib, beta 30kD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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