Rabbit MAP3K1 Polyclonal Antibody | anti-MAP3K1 antibody

MAP3K1 (Phospho-Thr1402) Antibody

Cat. Num. AAA9508632

• Gene Names: MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: MAP3K1; Polyclonal Antibody; MAP3K1 (Phospho-Thr1402) Antibody; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; anti-MAP3K1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: MAP3K1 (Phospho-Thr1402) Antibody detects endogenous levels of MAP3K1 only when phosphorylated at Thr1402.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1,512

• Applicable Applications for anti-MAP3K1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:T1402; M:T1381; R:T1381
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human MAP3K1 around the phosphorylation site of Thr1402.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP3K1 (Phospho-Thr1402) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from Jurkat cells, using MAP3K1 (Phospho-Thr1402) Antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 153945765
• NCBI GeneID: 4214
• NCBI Accession #: NP_005912.1
• NCBI GenBank Nucleotide #: NM_005921.1
• UniProt Accession #: Q13233
• Molecular Weight: 161 kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 1
• NCBI Official Symbol: MAP3K1
• NCBI Official Synonym Symbols: MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 1
• Protein Family: MAP3K12-binding inhibitory protein
• UniProt Gene Name: MAP3K1
• UniProt Synonym Gene Names: MAPKKK1; MEKK; MEKK1; MEK kinase 1; MEKK 1

NCBI Description

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]

Uniprot Description

Component of a protein kinase signal transduction cascade (PubMed:9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed:9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed:17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed:9808624).