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Western Blot (WB) (PLA2G2C Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PLA2G2C antibody detected the PLA2G2C protein (arrow).)

Rabbit anti-Human PLA2G2C Polyclonal Antibody | anti-Pla2g2c antibody

PLA2G2C, CT (PLA2G2C, Putative inactive group IIC secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase-like protein GIIC)

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
PLA2G2C; Polyclonal Antibody; CT (PLA2G2C; Putative inactive group IIC secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase-like protein GIIC); Anti -PLA2G2C; anti-Pla2g2c antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Pla2g2c antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunogen
PLA2G2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the C-terminal region of human PLA2G2C.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PLA2G2C Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PLA2G2C antibody detected the PLA2G2C protein (arrow).)

Western Blot (WB) (PLA2G2C Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PLA2G2C antibody detected the PLA2G2C protein (arrow).)

Immunohistochemistry (IHC)

(PLA2G2C Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLA2G2C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PLA2G2C Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLA2G2C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PLA2G2C Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PLA2G2C Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-Pla2g2c antibody
PLA2G2C is a inactive phospholipase (Probable).
Product Categories/Family for anti-Pla2g2c antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
16,919 Da
NCBI Official Full Name
Pla2g2c protein
NCBI Official Synonym Full Names
phospholipase A2, group IIC
NCBI Official Symbol
Pla2g2c
NCBI Protein Information
group IIC secretory phospholipase A2; PLA2-8; sPLA2-IIC; GIIC sPLA2; 14 kDa phospholipase A2; phospholipase A2, group 2C; phosphatidylcholine 2-acylhydrolase 2C; phosphatidylcholine 2-acylhydrolase GIIC
UniProt Protein Name
Group IIC secretory phospholipase A2
UniProt Gene Name
Pla2g2c
UniProt Synonym Gene Names
GIIC sPLA2; sPLA2-IIC
UniProt Entry Name
PA2GC_RAT

NCBI Description

catalyzes Ca+2 dependent hydrolysis of phospholipids including hydrolysis of L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol [RGD, Feb 2006]

Uniprot Description

Function: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Cofactor: Binds 1 calcium ion per subunit

By similarity.

Subcellular location: Secreted

Potential.

Sequence similarities: Belongs to the phospholipase A2 family.

Sequence caution: The sequence AAA57473.1 differs from that shown. Reason: Erroneous initiation.

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Product Notes

The Pla2g2c pla2g2c (Catalog #AAA6011153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLA2G2C, CT (PLA2G2C, Putative inactive group IIC secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase-like protein GIIC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G2C can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Pla2g2c pla2g2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G2C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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