Rabbit anti-Human, Mouse PLA2G1B Polyclonal Antibody | anti-PLA2G1B antibody

PLA2G1B, CT (PLA2G1B, PLA2, PLA2A, PPLA2, Phospholipase A2, Group IB phospholipase A2, Phosphatidylcholine 2-acylhydrolase 1B) (APC)

Cat. Num. AAA6334521

• Gene Names: PLA2G1B; PLA2; PLA2A; PPLA2
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PLA2G1B; Polyclonal Antibody; CT (PLA2G1B; PLA2; PLA2A; PPLA2; Phospholipase A2; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B) (APC); Phosphatidylcholine 2-acylhydrolase 1B; anti-PLA2G1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLA2G1B. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PLA2G1B antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 116-145 in the C-terminal region of human PLA2G1B, UniProt Accession #P04054.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6008455 in mouse spleen tissue lysates (35ug/lane). PLA2G1B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western Blot analysis of PLA2G1B (arrow) usingMBS6008455. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G1B gene.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells (right histogram) using MBS6008455 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PLA2G1B antibody

PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets

Product Categories/Family for anti-PLA2G1B antibody

Antibodies; Enzymes; Phospholipase

References

1. Xu, W., et al. J. Biol. Chem. 284(24):16659-16666(2009). 2. Han, C., et al. J. Cell. Biochem. 105(2):534-545(2008). 3. Kao, W.T., et al. Lipids Health Dis 7, 20 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4505847
• NCBI GeneID: 5319
• NCBI Accession #: NP_000919.1
• NCBI GenBank Nucleotide #: NM_000928.2
• UniProt Accession #: P04054; Q3KPI1; B2R4H5
• Molecular Weight: 16,360 Da
• NCBI Official Full Name: phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IB (pancreas)
• NCBI Official Symbol: PLA2G1B
• NCBI Official Synonym Symbols: PLA2; PLA2A; PPLA2
• NCBI Protein Information: phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B
• UniProt Protein Name: Phospholipase A2
• Protein Family: Phospholipase
• UniProt Gene Name: PLA2G1B
• UniProt Synonym Gene Names: PLA2; PLA2A; PPLA2
• UniProt Entry Name: PA21B_HUMAN

NCBI Description

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

Uniprot Description

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Secreted; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Phospholipase; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; DNA replication; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space; cell surface; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; bile acid binding; calcium ion binding; receptor binding

Biological Process: positive regulation of immune response; activation of MAPK activity; neutrophil mediated immunity; activation of phospholipase A2; glucose transport; signal transduction; activation of NF-kappaB transcription factor; phosphatidylcholine metabolic process; positive regulation of fibroblast proliferation; phospholipid metabolic process; fatty acid biosynthetic process; neutrophil chemotaxis; multicellular organismal lipid catabolic process; glycerophospholipid biosynthetic process; actin filament organization; arachidonic acid secretion; leukotriene biosynthetic process; defense response to Gram-positive bacterium; cellular response to insulin stimulus; innate immune response in mucosa; positive regulation of protein secretion; antibacterial humoral response; phosphatidic acid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; interleukin-8 production; positive regulation of DNA replication