PLA2G1B active protein

PLA2G1B Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120527

• Gene Names: PLA2G1B; PLA2; PLA2A; PPLA2
• Purity: >92% as determined by SDS-PAGE
• Synonyms: PLA2G1B; PLA2G1B Protein; Human; Recombinant (His Tag); Human PLA2G1B/PLA2 Protein (His Tag); PLA2 Protein; PLA2A Protein; PPLA2 Protein; phospholipase A2; group IB (pancreas); PLA2G1B active protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 10mM Tris, 5mM CaCl2, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser148

• Species: Human
• Activity: Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol. The specific activity is >2,500 pmol/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 16
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PLA2G1B (NP_000919.1)(Met 1-Ser 148) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PLA2G1B active protein

Background: phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein that belongs to the phospholipase A2 family. Phospholipase A2/PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2/PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2/PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2/PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.

References

Labonta©,E.D. et al., 2006, Diabetes. 55 (4) :935-41. Mounier,C.M. et al.,2008, Br J Cancer. 98 (3):587-95. Hui,D.Y. et al., 2009, Br J Pharmacol. 157 (7):1263-9. Labonta©,E.D. et al., 2010, FASEB J. 24 (7):2516-24.

NCBI and Uniprot Product Information

• NCBI GI #: 4505847
• NCBI GeneID: 5319
• NCBI Accession #: NP_000919.1
• NCBI GenBank Nucleotide #: NM_000928.2
• UniProt Accession #: P04054; Q3KPI1; B2R4H5
• Molecular Weight: 16,360 Da
• NCBI Official Full Name: phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IB (pancreas)
• NCBI Official Symbol: PLA2G1B
• NCBI Official Synonym Symbols: PLA2; PLA2A; PPLA2
• NCBI Protein Information: phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B
• UniProt Protein Name: Phospholipase A2
• Protein Family: Phospholipase
• UniProt Gene Name: PLA2G1B
• UniProt Synonym Gene Names: PLA2; PLA2A; PPLA2
• UniProt Entry Name: PA21B_HUMAN

NCBI Description

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

Uniprot Description

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Secreted; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Phospholipase; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; DNA replication; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space; cell surface; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; bile acid binding; calcium ion binding; receptor binding

Biological Process: positive regulation of immune response; activation of MAPK activity; neutrophil mediated immunity; activation of phospholipase A2; glucose transport; signal transduction; activation of NF-kappaB transcription factor; phosphatidylcholine metabolic process; positive regulation of fibroblast proliferation; phospholipid metabolic process; fatty acid biosynthetic process; neutrophil chemotaxis; multicellular organismal lipid catabolic process; glycerophospholipid biosynthetic process; actin filament organization; arachidonic acid secretion; leukotriene biosynthetic process; defense response to Gram-positive bacterium; cellular response to insulin stimulus; innate immune response in mucosa; positive regulation of protein secretion; antibacterial humoral response; phosphatidic acid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; interleukin-8 production; positive regulation of DNA replication