Sheep anti-Human PLA2G16 Polyclonal Antibody | anti-PLA2G16 antibody

PLA2G16 (Group XVI Phospholipase A2, Adipose-specific Phospholipase A2, AdPLA, H-rev 107 Protein Homolog, HRAS-like Suppressor 3, HREV107, HREV107-3, Renal Carcinoma Antigen NY-REN-65, HRASLS3)

Cat. Num. AAA630473

• Gene Names: PLA2G16; AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; MGC118754; H-REV107-1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PLA2G16; Polyclonal Antibody; PLA2G16 (Group XVI Phospholipase A2; Adipose-specific Phospholipase A2; AdPLA; H-rev 107 Protein Homolog; HRAS-like Suppressor 3; HREV107; HREV107-3; Renal Carcinoma Antigen NY-REN-65; HRASLS3); Anti -PLA2G16 (Group XVI Phospholipase A2; anti-PLA2G16 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLA2G16.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in 100ul sterile water.

• Applicable Applications for anti-PLA2G16 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA:0.5-1ug/ml; Western Blot:1ug/ml
• Immunogen: Purified, E.coli-derived recombinant human PLA2G16. (Asp12-Asp132; Accession #P53816).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PLA2G16 antibody

PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca++-dependent 17-18kD member of the H-rev107 family of molecules. It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids. The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels. This results in decreased hormone-sensitive lipase activity and increased adiposity. Human PLA216 is 16211aa in length. It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa134-154. The enzymatic activity resides in His23 and Cys113. There is one potential isoform that shows a 20aa substitution for the first four amino acids. Over aa12-132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.

Product Categories/Family for anti-PLA2G16 antibody

Antibodies; Abs to Enzymes; Phospholipase

NCBI and Uniprot Product Information

• NCBI GI #: 75517991
• NCBI GeneID: 11145
• UniProt Accession #: Q3MI98; P53816
• Molecular Weight: 21,441 Da
• NCBI Official Full Name: PLA2G16 protein
• NCBI Official Synonym Full Names: phospholipase A2, group XVI
• NCBI Official Symbol: PLA2G16
• NCBI Official Synonym Symbols: AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; MGC118754; H-REV107-1
• NCBI Protein Information: group XVI phospholipase A2; OTTHUMP00000237069; adipose-specific PLA2; HRAS-like suppressor 1; HRAS-like suppressor 3; H-rev 107 protein homolog; Ca-independent phospholipase A1/2; adipose-specific phospholipase A2; renal carcinoma antigen NY-REN-65
• UniProt Protein Name: PLA2G16 protein
• Protein Family: HRAS-like suppressor
• UniProt Gene Name: PLA2G16
• UniProt Entry Name: Q3MI98_HUMAN

Uniprot Description

PLA2G16: Exhibits PLA1/2 activity, catalyzing the calcium- independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. N- and O- acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity. Belongs to the H-rev107 family.

Protein type: EC 3.1.1.4; EC 3.1.1.32; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; cytosol

Molecular Function: phospholipase A2 activity; protein binding; phospholipase A1 activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; lipid catabolic process; negative regulation of cell cycle