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Western Blot (WB)
(Western Blot analysis of 3T3, K562 cells using Aldolase A Polyclonal Antibody at dilution of 1:1000.)
Glycolysis II Antibody Kit
Glycolysis II Antibody Sampler Kit
Applications
Western Blot
Synonyms
Glycolysis II; Glycolysis II Antibody Sampler Kit; Glycolysis II antibody kit
Applicable Applications for Glycolysis II antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of 3T3, K562 cells using Aldolase A Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 3T3, K562 cells using Aldolase A Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using ENO1 Polyclonal Antibody at dilution of 1:600)
Western Blot (WB)
(Western Blot analysis of Hela cells using ENO1 Polyclonal Antibody at dilution of 1:600)
Western Blot (WB)
(Western Blot analysis of SH-SY5Y cells using Enolase Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of SH-SY5Y cells using Enolase Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Hela cells with PDK1 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Hela cells with PDK1 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 293 cells with PFK-2 car Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 293 cells with PFK-2 car Polyclonal Antibody.)
Related Product Information for Glycolysis II antibody kit
Background: Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis. The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified. Aldolase (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis. Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (alpha-enolase), enolase-2 (gamma-enolase) and enolase-3 (beta-enolase) that can form both homo- and heterodimers. Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form.
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Product Notes
The Glycolysis II (Catalog #AAA2550098) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glycolysis II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Glycolysis II for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycolysis II, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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