Rabbit anti-Human PKM2 Polyclonal Antibody | anti-PKM2 antibody

PKM2 Polyclonal Antibody

Cat. Num. AAA3013271

• Gene Names: PKM; PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: Peptide affinity purified.
• Synonyms: PKM2; Polyclonal Antibody; PKM2 Polyclonal Antibody; CTHBP; OIP-3; THBP1; PKM; OIP3; PK2; PK3; Cytosolic thyroid hormone binding protein; Cytosolic thyroid hormone-binding protein; KPYM; MGC3932; OIP 3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK muscle type; PK; muscle type; pykm; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; pyruvate kinase PKM; Pyruvate kinase; muscle 2; TCB; Thyroid hormone binding protein 1; Thyroid hormone binding protein cytosolic; Thyroid hormone-binding protein 1; Tumor M2 PK; Tumor M2-PK; anti-PKM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PKM2 polyclonal antibody detects endogenous levels of PKM2 protein.
• Purity/Purification: Peptide affinity purified.
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 605

• Applicable Applications for anti-PKM2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1,000; ICC: 1:100-1:500; IHC: 1:50-1:200
• Immunogen: Synthetic peptide within human PKM2 aa 380-430.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of PKM2 on 293 cell lysate using anti-PKM2 antibody at 1/500 dilution.)

Immunocytochemistry (ICC)

(ICC staining PKM2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-PKM2 antibody

In mammals, four different isoenzymes exist for pyruvate kinase. Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKM (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1, 6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. The M2-type isoform also interacts with Oct-4 via its C-terminal domain, functioning to enhance Oct-4 transcriptional activity. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.

NCBI and Uniprot Product Information

• NCBI GI #: 332164775
• NCBI GeneID: 5315
• NCBI Accession #: NP_001193725.1
• NCBI GenBank Nucleotide #: NP_001193725.1
• UniProt Accession #: P14618
• Molecular Weight: 58kDa
• NCBI Official Full Name: pyruvate kinase PKM isoform c
• NCBI Official Synonym Full Names: pyruvate kinase M1/2
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
• UniProt Entry Name: KPYM_HUMAN

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]