Rabbit anti-Human PKM1/2 Polyclonal Antibody | anti-PKM1/2 antibody

PKM1/2 Antibody

Cat. Num. AAA9404828

• Gene Names: PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: PKM1/2; Polyclonal Antibody; PKM1/2 Antibody; PKM; PK3; OIP3; PK2; anti-PKM1/2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total PKM1/2 protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 605

• Applicable Applications for anti-PKM1/2 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa. 36~40(D-S-P-P-I) derived from Human PKM1/2.
• Target Name: PKM1/2
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 and 293 cells using PKM1/2 Antibody.)

Related Product Information for anti-PKM1/2 antibody

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Product Categories/Family for anti-PKM1/2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 332164775
• NCBI GeneID: 5315
• NCBI Accession #: NP_001193725.1
• NCBI GenBank Nucleotide #: NM_001206796.1
• UniProt Accession #: P14618; P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
• Molecular Weight: 56,273 Da
• NCBI Official Full Name: pyruvate kinase PKM isoform c
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
• UniProt Entry Name: KPYM_HUMAN

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Uniprot Description

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.