Human mannose receptor, C type 2 ELISA Kit | MRC2 elisa kit

Human C-type mannose receptor 2, MRC2 ELISA Kit

Cat. Num. AAA946449

• Gene Names: MRC2; CD280; UPARAP; CLEC13E; ENDO180
• Reactivity: Human
• Synonyms: mannose receptor; C type 2; Human C-type mannose receptor 2; MRC2 ELISA Kit; Human C-type mannose receptor 2 (MRC2) ELISA kit; CD280; CLEC13E; ENDO180; FLJ35911; KIAA0709; UPARAP; endocytic receptor (macrophage mannose receptor family) ; urokinase plasminogen activator receptor-associated protein; MRC2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MRC2. No significant cross-reactivity or interference between human MRC2 and analogues was observed.
• Sequence Length: 1479

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of human MRC2 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRC2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRC2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110624774
• NCBI GeneID: 9902
• NCBI Accession #: NP_006030.2
• NCBI GenBank Nucleotide #: NM_006039.4
• UniProt Accession #: Q9UBG0; Q7LGE7; Q9Y5P9; A6H8K4; D3DU08
• Molecular Weight: 166,674 Da
• NCBI Official Full Name: C-type mannose receptor 2
• NCBI Official Synonym Full Names: mannose receptor, C type 2
• NCBI Official Symbol: MRC2
• NCBI Official Synonym Symbols: CD280; UPARAP; CLEC13E; ENDO180
• NCBI Protein Information: C-type mannose receptor 2; endocytic receptor 180; UPAR-associated protein; macrophage mannose receptor 2; C-type lectin domain family 13 member E; endocytic receptor (macrophage mannose receptor family); urokinase-type plasminogen activator receptor-associated protein
• UniProt Protein Name: C-type mannose receptor 2
• Protein Family: C-type mannose receptor
• UniProt Gene Name: MRC2
• UniProt Synonym Gene Names: CLEC13E; ENDO180; KIAA0709; UPARAP; UPAR-associated protein; Urokinase receptor-associated protein
• UniProt Entry Name: MRC2_HUMAN

NCBI Description

This gene encodes a member of the mannose receptor family of proteins that contain a fibronectin type II domain and multiple C-type lectin-like domains. The encoded protein plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. Expression of this gene may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease. [provided by RefSeq, Feb 2012]

Uniprot Description

MRC2: May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: focal adhesion; membrane; integral to membrane

Molecular Function: collagen binding; protein binding; carbohydrate binding

Biological Process: osteoblast differentiation; collagen catabolic process; endocytosis