Rabbit PKD1 Polyclonal Antibody | anti-PKD1 antibody

PKD1 (G459) Polyclonal Antibody

Cat. Num. AAA3001338

• Gene Names: PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PKD1; Polyclonal Antibody; PKD1 (G459) Polyclonal Antibody; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu; PRKD1; PKD; PRKCM; PKCu; PKC u; PKC-u; PKC mu; PKCmu; anti-PKD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PKD1 (G459) polyclonal antibody detects endogenous levels of PKD1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 912

• Applicable Applications for anti-PKD1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 424-482 of Human PKD1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PKD1 (G459) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-PKD1 antibody

Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. Protein kinase D (PKD), also called PKCmu, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway. In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCu. Phosphorylation at Ser916 correlates with PKD/PKCu catalytic activity.

NCBI and Uniprot Product Information

• NCBI GI #: 115529463
• NCBI GeneID: 5587
• NCBI Accession #: NP_002733.2
• NCBI GenBank Nucleotide #: NP_002733.2
• UniProt Accession #: Q15139
• Molecular Weight: ~ 115kDa
• NCBI Official Full Name: serine/threonine-protein kinase D1 isoform 2
• NCBI Official Synonym Full Names: protein kinase D1
• NCBI Official Symbol: PRKD1
• NCBI Official Synonym Symbols: PKD; PKCM; CHDED; PRKCM; PKC-MU
• NCBI Protein Information: serine/threonine-protein kinase D1
• UniProt Protein Name: Serine/threonine-protein kinase D1
• Protein Family: Polycystin
• UniProt Gene Name: PRKD1
• UniProt Synonym Gene Names: PKD; PKD1; PRKCM
• UniProt Entry Name: KPCD1_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]

Uniprot Description

PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A

Protein type: Kinase, protein; Autophagy; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 2.7.11.13; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 14q11

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; intercellular junction; trans-Golgi network; cell cortex; nucleus; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding

Biological Process: integrin-mediated signaling pathway; sphingolipid metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid biosynthetic process; apoptosis; protein amino acid autophosphorylation; negative regulation of endocytosis; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; signal transduction; positive regulation of peptidyl-serine phosphorylation; activation of NF-kappaB transcription factor; Golgi vesicle transport; cell proliferation; positive regulation of osteoblast differentiation; peptidyl-serine phosphorylation; positive regulation of angiogenesis; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; inflammatory response; Golgi organization and biogenesis