Rabbit anti-Human, Mouse PKC zeta Polyclonal Antibody | anti-PKC zeta antibody

PKC zeta (Phospho-Thr560) Antibody

Cat. Num. AAA9412001

• Gene Names: PRKCZ; PKC2; PKC-ZETA
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: PKC zeta; Polyclonal Antibody; PKC zeta (Phospho-Thr560) Antibody; KPCZ; PKC-zeta; PKC2; PRKCZ; nPKC-zeta; anti-PKC zeta antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of PKC zeta only when phosphorylated at threonine 560.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 409

• Applicable Applications for anti-PKC zeta antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phospho rylation site of threonine560(Q-L-T(p)-P-D) derived from Human PKC zeta.
• Modification: Phospho-Thr560
• Target Name: PKC zeta
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from COS7 cells treated with PMA using Phospho-PKC zeta (Thr560) antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-PKC zeta antibody

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Categories/Family for anti-PKC zeta antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 75709226
• NCBI GeneID: 5590
• NCBI Accession #: NP_001028753.1
• NCBI GenBank Nucleotide #: NM_001033581.1
• UniProt Accession #: Q05513; Q15207; Q5SYT5; Q969S4; A8K4N0; A8MU64; B7Z2J7; E9PCW2
• Molecular Weight: 56,136 Da
• NCBI Official Full Name: protein kinase C zeta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C, zeta
• NCBI Official Symbol: PRKCZ
• NCBI Official Synonym Symbols: PKC2; PKC-ZETA
• NCBI Protein Information: protein kinase C zeta type
• UniProt Protein Name: Protein kinase C zeta type
• UniProt Gene Name: PRKCZ
• UniProt Synonym Gene Names: PKC2
• UniProt Entry Name: KPCZ_HUMAN

NCBI Description

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; AGC group; PKC family; Iota subfamily

Chromosomal Location of Human Ortholog: 1p36.33-p36.2

Cellular Component: nuclear matrix; tight junction; apical cortex; leading edge; nuclear envelope; intercellular junction; cytosol; lipid raft; axon hillock; filamentous actin; membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; plasma membrane; microtubule organizing center; cell junction; endosome

Molecular Function: protein serine/threonine kinase activity; protein domain specific binding; protein binding; potassium channel regulator activity; phospholipase binding; protein kinase C activity; insulin receptor substrate binding; metal ion binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: protein heterooligomerization; membrane hyperpolarization; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of interleukin-4 production; positive regulation of glucose import; positive regulation of T-helper 2 cell differentiation; negative regulation of hydrolase activity; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; vesicle transport along microtubule; inflammatory response; positive regulation of cell-matrix adhesion; phospholipase D activation; negative regulation of peptidyl-tyrosine phosphorylation; platelet activation; cell migration; activation of protein kinase B; microtubule cytoskeleton organization and biogenesis; peptidyl-serine phosphorylation; long-term memory; establishment of cell polarity; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; blood coagulation; vascular endothelial growth factor receptor signaling pathway; negative regulation of protein complex assembly; negative regulation of apoptosis