Western Blot (WB)
Rabbit anti-Human CD80 / B7-1 Polyclonal Antibody | anti-CD80 / B7-1 antibody
Anti-CD80 / B7-1 antibody
Reactivity
Human
Applications
Western Blot
Purity
Immunogen affinity purified
Synonyms
CD80 / B7-1; Polyclonal Antibody; Anti-CD80 / B7-1 antibody; Polyclonal Anti- CD80 / B7-1; anti-CD80 / B7-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid
Applicable Applications for anti-CD80 / B7-1 antibody
Western Blot (WB)
Application Notes
WB (1:500)
Immunogen
A synthetic peptide (conjugated to KLH) corresponding to extracellular region of human CD80 / B7-1.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 25% Glycerol and 0.05% Sodium Azide
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 25% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-CD80 / B7-1 antibody
Cluster of Differentiation 80 (CD80), also called B-lymphocyte activation antigen B7-1 (formerly referred to as B7), is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. The B7-related molecules CD80 and CD86 are expressed on antigen-presenting cells, bind the homologous T cell receptors CD28 and CTLA-4, and trigger costimulatory signals important for optimal T cell activation. All four molecules are immunoglobulin superfamily members, each comprising an extracellular Ig variable-like (IgV) domain, with CD80 and CD86 containing an additional Ig constant-like (IgC) domain.1 The upregulation of B7-1 in podocytes may contribute to the pathogenesis of proteinuria by disrupting the glomerular filter and provides a novel molecular target to tackle proteinuric kidney diseases. This suggests that B7-1 has a novel function in danger signaling by nonimmune cells.2
References
1. Peach, R J; Bajorath J, Naemura J, Leytze G, Greene J, Aruffo A, Linsley P S (Sep. 1995). "Both extracellular immunoglobin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28". J. Biol. Chem. (UNITED STATES) 270 (36): 21181-7. 2. Reiser, J., von Gersdorff, G., Loos, M., Oh, J., Asanuma, K., Giardino, L., Rastaldi, M. P., Calvaresi, N., Watanabe, H., Schwarz, K., Faul, C., Kretzler, M., Davidson, A., Sugimoto, H., Kalluri, R., Sharpe, A. H., Kreidberg, J. A., Mundel, P. Induction of B7-1 in podocytes is associated with nephrotic syndrome. J. Clin. Invest. 113: 1390-1397, 2004.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD80/B7-1 transmembrane form
NCBI Official Symbol
LOC554317
NCBI Protein Information
CD80/B7-1 transmembrane form
Research Articles on CD80 / B7-1
Similar Products
Product Notes
The CD80 / B7-1 (Catalog #AAA355088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD80 / B7-1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD80 / B7-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB (1:500). Researchers should empirically determine the suitability of the CD80 / B7-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD80 / B7-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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