Rabbit anti-Human PKC eta Polyclonal Antibody | anti-PRKCH antibody

PKC eta, NT (Protein Kinase C eta Type, nPKC-eta, PKC-L, PRKCH, PKCL, PRKCL) (APC)

Cat. Num. AAA6495214

• Gene Names: PRKCH; PKCL; PKC-L; PRKCL; nPKC-eta
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PKC eta; Polyclonal Antibody; NT (Protein Kinase C eta Type; nPKC-eta; PKC-L; PRKCH; PKCL; PRKCL) (APC); anti-PRKCH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKC.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PRKCH antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PKC (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PKC eta N-term Pab in NCI-H460 cell lysate. PKC eta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of PRKCH (arrow) using PKC eta Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCH gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PRKCH antibody

Antibodies; Protein Kinases

References

1. Palmer, R.H., et al., FEBS Lett. 356(1):5-8 (1994). 2. Bacher, N., et al., Mol. Cell. Biol. 11(1):126-133 (1991). 3. Bacher, N., et al., Mol. Cell. Biol. 12(3), 1404 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 28557781
• NCBI GeneID: 5583
• NCBI Accession #: NP_006246.2
• NCBI GenBank Nucleotide #: NM_006255.4
• UniProt Accession #: P24723; Q16246; Q8NE03; B4DJN5
• Molecular Weight: 59,521 Da
• NCBI Official Full Name: protein kinase C eta type
• NCBI Official Synonym Full Names: protein kinase C eta
• NCBI Official Symbol: PRKCH
• NCBI Official Synonym Symbols: PKCL; PKC-L; PRKCL; nPKC-eta
• NCBI Protein Information: protein kinase C eta type
• UniProt Protein Name: Protein kinase C eta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCH
• UniProt Synonym Gene Names: PKCL; PRKCL
• UniProt Entry Name: KPCL_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]

Uniprot Description

PKCH: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-p27Kip1 complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1- dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Interacts with FYN and RALA. Interacts with DGKQ. Most abundant in lung, less in heart and skin. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; AGC group; PKC family; Eta subfamily

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: cytoplasm; cytosol; intercellular junction; plasma membrane

Molecular Function: ATP binding; calcium-independent protein kinase C activity; enzyme binding; metal ion binding; protein kinase C activity; Ral GTPase binding

Biological Process: activation of NF-kappaB transcription factor; peptidyl-serine phosphorylation; platelet activation; positive regulation of B cell receptor signaling pathway; positive regulation of keratinocyte differentiation; protein amino acid phosphorylation; signal transduction

Disease: Stroke, Ischemic