TBK1 recombinant protein

TBK1, Active

Cat. Num. AAA517613

• Gene Names: TBK1; NAK; T2K; FTDALS4
• Applications: Western Blot
• Synonyms: TBK1; Active; NAK; T2K; FLJ11330; TBK1 recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for TBK1 recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for TBK1 recombinant protein

Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC034950.
TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases (1). Activation of IkB kinases allows the phosphorylation of IkB protein which is then degraded via the ubiquitination pathway. This mechanism allows the activation of the NFkB transcriptional complex. TBK1 is a specific upstream regulator of IkB kinases and can also interact and the IkB protein TANK. TBK1 is a component of the virus-activated kinase that phosphorylate IRF3 and IRF7 allowing their dimerization and translocation to the nucleus, where they induce transcription of interferon (2).

Product Categories/Family for TBK1 recombinant protein

Protein Kinases; Cytoplasmic Tyrosine Kinases; Inflammation; NfkB Pathway; Ser/Thr Kinases

References

1. Tojima, Y. et al: NAK is an I-kappa-B kinase-activating kinase. Nature 404: 778-782, 2000.
2. Sharma, S. et al: Triggering the interferon antiviral response through an IKK-related pathway. Science 300: 1148-1151, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 34192452
• NCBI GeneID: 29110
• Molecular Weight: 83,642 Da
• NCBI Official Full Name: Homo sapiens TANK-binding kinase 1, mRNA
• NCBI Official Synonym Full Names: TANK binding kinase 1
• NCBI Official Symbol: TBK1
• NCBI Official Synonym Symbols: NAK; T2K; FTDALS4
• NCBI Protein Information: serine/threonine-protein kinase TBK1
• Protein Family: Serine/threonine-protein kinase

NCBI Description

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]