Western Blot (WB)
(Western blot analysis of PKC alpha expression in MCF7 (A), NIH3T3 (B), mouse liver (C), PC2 (D) whole cell lysates.)
Rabbit PKC alpha Polyclonal Antibody | anti-PRKCA antibody
Anti-PKC alpha Antibody
Western Blot (WB)
(Western blot analysis of PKC alpha expression in MCF7 (A), NIH3T3 (B), mouse liver (C), PC2 (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PKC alpha expression in MCF7 (A), NIH3T3 (B), mouse liver (C), PC2 (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKC alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKC alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.
Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; AGC group; PKC family; Alpha subfamily
Chromosomal Location of Human Ortholog: 17q22-q23.2
Cellular Component: apical part of cell; cell soma; cytoplasm; cytosol; dendrite; endoplasmic reticulum; mitochondrial membrane; mitochondrion; nucleoplasm; perinuclear region of cytoplasm; photoreceptor outer segment; plasma membrane
Molecular Function: ATP binding; calcium-dependent protein kinase C activity; enzyme binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity; zinc ion binding
Biological Process: adenylate cyclase activation; angiogenesis; blood coagulation; cell adhesion; cellular calcium ion homeostasis; chondrocyte differentiation; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; gene expression; inactivation of MAPK activity; induction of positive chemotaxis; innate immune response; mitotic cell cycle; mitotic nuclear envelope disassembly; negative regulation of adenylate cyclase activity; negative regulation of cell proliferation; negative regulation of glucose import; negative regulation of insulin receptor signaling pathway; nerve growth factor receptor signaling pathway; neutrophil chemotaxis; peptidyl-serine phosphorylation; phospholipase C activation; phototransduction, visible light; platelet activation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of inflammatory response; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of macrophage differentiation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of insulin secretion; regulation of mRNA stability; regulation of muscle contraction; regulation of peptidyl-tyrosine phosphorylation; regulation of rhodopsin mediated signaling; regulation of the force of heart contraction; rhodopsin mediated signaling; signal transduction; synaptic transmission; vascular endothelial growth factor receptor signaling pathway
Research Articles on PRKCA
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Product Notes
The PRKCA prkca (Catalog #AAA8242659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKC alpha Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's PKC alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the PRKCA prkca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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