Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Enoyl-CoA delta isomerase 1, mitochondrial (DCI) Standard Curve.)
Human Enoyl-CoA delta Isomerase 1, Mitochondrial (DCI) ELISA Kit | DCI elisa kit
Human Enoyl-CoA delta Isomerase 1, Mitochondrial (DCI) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Enoyl-CoA delta isomerase 1, mitochondrial (DCI) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Enoyl-CoA delta isomerase 1, mitochondrial (DCI) Standard Curve.)
Background: ECI1 encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
Uniprot Description
DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 5.3.3.8; Isomerase; Mitochondrial; Lipid Metabolism - fatty acid
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane
Molecular Function: dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity
Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process
Research Articles on DCI
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Product Notes
The Human DCI eci1 (Catalog #AAA9715796) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715796 ELISA Kit recognizes Human DCI. It is sometimes possible for the material contained within the vial of "Enoyl-CoA delta Isomerase 1, Mitochondrial (DCI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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