Rabbit anti-Human, mouse PKA C-alpha/PKA C-beta Polyclonal Antibody | anti-PRKACA antibody

PKA C-alpha/PKA C-beta Antibody (N-term)

Cat. Num. AAA9208234

• Gene Names: PRKACA; PKACA; PPNAD4
• Reactivity: Human, mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PKA C-alpha/PKA C-beta; Polyclonal Antibody; PKA C-alpha/PKA C-beta Antibody (N-term); cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA; PKACA; anti-PRKACA antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PKA C-alpha/PKA C-beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-40 amino acids from the N-terminal region of human PKA C-alpha/PKA C-beta.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 9-40
• Sequence Length: 343

• Applicable Applications for anti-PRKACA antibody: WB, IHC-P, E
• Application Notes: WB~~1:1000; IHC-P~~1:50~100
• Antigen Region: 9-40
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

Western Blot (WB)

(Anti-PKA-C-alpha/beta Antibody at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PRKACA antibody

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by the PRKACA gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase.

Product Categories/Family for anti-PRKACA antibody

Cancer; Metabolism; Signal Transduction

References

Tateyama, M., et al., J. Biol. Chem. 278(47):46718-46726 (2003).
Cartier, C., et al., J. Biol. Chem. 278(37):35211-35219 (2003).
Libersan, D., et al., Thromb. Haemost. 89(2):310-317 (2003).
Yasuda, H., et al., Nat. Neurosci. 6(1):15-16 (2003).
Kaneider, N.C., et al., J. Bone Miner. Res. 17(10):1872-1882 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4506055
• NCBI GeneID: 5566
• NCBI Accession #: NP_002721.1; NP_997401.1
• NCBI GenBank Nucleotide #: NM_002730.3
• UniProt Accession #: P17612; Q32P54; Q9H2Y0; Q9NRB4; Q9NRH9
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, catalytic, alpha
• NCBI Official Symbol: PRKACA
• NCBI Official Synonym Symbols: PKACA; PPNAD4
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKACA
• UniProt Synonym Gene Names: PKACA; PKA C-alpha
• UniProt Entry Name: KAPCA_HUMAN

NCBI Description

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

Uniprot Description

PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.11; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; membrane; mitochondrion; plasma membrane; nucleus; neuromuscular junction; cytosol; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; protein kinase binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; water transport; pathogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; triacylglycerol catabolic process; G2/M transition of mitotic cell cycle; transmembrane transport; sperm capacitation; epidermal growth factor receptor signaling pathway; regulation of synaptic transmission; fibroblast growth factor receptor signaling pathway; cytosolic calcium ion homeostasis; regulation of osteoblast differentiation; regulation of heart rate; organelle organization and biogenesis; activation of protein kinase A; peptidyl-threonine phosphorylation; glucose metabolic process; gluconeogenesis; peptidyl-serine phosphorylation; mesoderm formation; phospholipase C activation; regulation of protein binding; neural tube closure; carbohydrate metabolic process; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; regulation of insulin secretion