Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
Rabbit PKA Polyclonal Antibody | anti-PKA antibody
PKA antibody (Thr197)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
PKA; Polyclonal Antibody; PKA antibody (Thr197); Polyclonal PKA; Anti-PKA; cAMP-dependent protein kinase catalytic subunit alpha; isoform 1; KAPCA; MGC102831; MGC48865; PKA C-alpha; PKACA; PRKACA; protein kinase A catalytic subunit; protein kinase; cAMP-dependent; catalytic; alpha; anti-PKA antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
PKA alpha/ beta CAT antibody detects endogenous levels of PKA alpha/ beta CAT only when phosphorylated at threonine 197
Form/Format
Supplied in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
342
Applicable Applications for anti-PKA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:10000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:10000
Biological Significance
PKA is a catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits.
Cross-Reactivity
Mouse, Rat. Specific detection of phosphorylated Human: Thr197, Mouse: Thr197, Rat: Thr197
Immunogen
PKA antibody was raised in Rabbit using synthesized phosphopeptide derived from human PKA around the phosphorylation site of threonine 197 as the immunogen
Preparation and Storage
Store at -20 degree C for 1 year
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain cells, using PKA alpha/beta CAT (Phospho-Thr197) antibody)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain cells, using PKA alpha/beta CAT (Phospho-Thr197) antibody)
Related Product Information for anti-PKA antibody
Purified Rabbit polyclonal PKA antibody (Thr197)
Product Categories/Family for anti-PKA antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
40,225 Da
NCBI Official Full Name
PKA
UniProt Protein Name
PKA protein
Protein Family
UniProt Gene Name
PKA
UniProt Entry Name
O44020_PLAFA
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Product Notes
The PKA pka (Catalog #AAA839455) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PKA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:100-1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the PKA pka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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