Western Blot (WB)
(Western blot analysis of PKA expression in lysates from Jurkat cells (Lane 1 & 2), mouse small intestine (Lane 3) and rat kidney tissue (Lane 4).)
Rabbit PKA Polyclonal Antibody | anti-PKA antibody
PKA antibody
Applications
Immunoprecipitation, Western Blot
Purity
PKA antibody was purified by affinity chromatography
Synonyms
PKA; Polyclonal Antibody; PKA antibody; Polyclonal PKA; anti-PKA; PRKACA; PKACA; MGC102831; MGC48865; anti-PKA antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
PKA antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
Concentration
500 ug/ml (varies by lot)
Sequence Length
342
Applicable Applications for anti-PKA antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 20-30 ug/ml
WB: 0.5-4 ug/ml
WB: 0.5-4 ug/ml
Biological Significance
Protein kinase A also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.
Cross-Reactivity
Human, Mouse, Rat, Bovine
Immunogen
PKA antibody was raised in Rabbit using synthetic peptide surrounding amino acid 11 of the catalytic subunit of human PKA alpha isoform as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of PKA expression in lysates from Jurkat cells (Lane 1 & 2), mouse small intestine (Lane 3) and rat kidney tissue (Lane 4).)
Western Blot (WB)
(Western blot analysis of PKA expression in lysates from Jurkat cells (Lane 1 & 2), mouse small intestine (Lane 3) and rat kidney tissue (Lane 4).)
Related Product Information for anti-PKA antibody
Rabbit polyclonal PKA antibody
Product Categories/Family for anti-PKA antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
40,225 Da
NCBI Official Full Name
PKA
UniProt Protein Name
PKA protein
Protein Family
UniProt Gene Name
PKA
UniProt Entry Name
O44020_PLAFA
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Product Notes
The PKA pka (Catalog #AAA837651) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PKA can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). IP: 20-30 ug/ml WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the PKA pka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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