Rabbit PKA Polyclonal Antibody | anti-PKA antibody

PKA Antibody

Cat. Num. AAA841366

• Gene Names: Prkaca; PKCD; Pkaca
• Reactivity: Human, Mouse, Rat, Bovine, Sheep, Pig
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: PKA; Polyclonal Antibody; PKA Antibody; PRKACA; MGC102831; PKACA; MGC48865; anti-PKA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Sheep, Pig
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the 40 kDa PKA of human, mouse, rat, bovine, sheep, and pig origins on SDS-PAGE immunoblots. Blocking peptide (MBS841366) is available separately.
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-PKA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 343

• Applicable Applications for anti-PKA antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western blot analysis (0.5-4 ug/ml). Jurkat cell lysate (Please Inquire) can be used as a positive control. Based on researcher’s feedback, it can also be used in Immunoprecipitation (20-30 ug/ml). However, the optimal conditions should be determined individually.
• Immunogen: Synthetic peptide surrounding amino acid 11 of the catalytic subunit of human PKA alpha isoform
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: Store at -20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Testing Data

(MBS841366)

Related Product Information for anti-PKA antibody

Background: Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. It has been smuggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.

NCBI and Uniprot Product Information

• NCBI GI #: 483968030
• NCBI GeneID: 18747
• NCBI Accession #: NP_001264827.1
• NCBI GenBank Nucleotide #: NM_001277898.1
• UniProt Accession #: P05132; Q9JID0
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform 2
• NCBI Official Synonym Full Names: protein kinase, cAMP dependent, catalytic, alpha
• NCBI Official Symbol: Prkaca
• NCBI Official Synonym Symbols: PKCD; Pkaca
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Protein Family: Protein lysine acetyltransferase
• UniProt Gene Name: Prkaca
• UniProt Synonym Gene Names: Pkaca; PKA C-alpha
• UniProt Entry Name: KAPCA_MOUSE

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013]

Uniprot Description

PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.

Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.11; AGC group; PKA family

Cellular Component: Golgi apparatus; centrosome; neuron projection; protein complex; mitochondrion; cilium; cAMP-dependent protein kinase complex; cell projection; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; neuromuscular junction; AMP-activated protein kinase complex

Molecular Function: cAMP-dependent protein kinase activity; nucleotide binding; protein kinase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; protein complex binding; kinase activity; ATP binding; Rab GTPase binding

Biological Process: regulation of synaptic transmission; regulation of synaptic transmission, glutamatergic; regulation of osteoblast differentiation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; negative regulation of meiotic cell cycle; protein amino acid phosphorylation; regulation of cellular respiration; positive regulation of protein export from nucleus; peptidyl-serine phosphorylation; mesoderm formation; neural tube closure; positive regulation of cell proliferation; phosphorylation; sperm capacitation