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Western Blot (WB) (All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Hela whole cell lysateLane 4: MOLT-4 whole cell lysateLane 5: U-251 MG whole cell lysateLane 6: mouse brain lysateLane 7: rat brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit PITPNA Polyclonal Antibody | anti-PITPNA antibody

PITPNA Antibody (C-term)

Gene Names
PITPNA; PITPN; VIB1A; HEL-S-36; PI-TPalpha
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Rabbit
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
PITPNA; Polyclonal Antibody; PITPNA Antibody (C-term); Phosphatidylinositol transfer protein alpha isoform; PITPN; anti-PITPNA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Rabbit
Clonality
Polyclonal
Isotype
Ig
Specificity
This PITPNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the C-terminal region of human PITPNA.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
270
Applicable Applications for anti-PITPNA antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:1000
IHC-P: 1:25
Antigen Source
Human
Antigen Region
243-270
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Hela whole cell lysateLane 4: MOLT-4 whole cell lysateLane 5: U-251 MG whole cell lysateLane 6: mouse brain lysateLane 7: rat brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Hela whole cell lysateLane 4: MOLT-4 whole cell lysateLane 5: U-251 MG whole cell lysateLane 6: mouse brain lysateLane 7: rat brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: MOLT-4 whole cell lysateLane 3: Hela whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: mouse brain lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: MOLT-4 whole cell lysateLane 3: Hela whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: mouse brain lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of lysates from Hela cell line,mouse brain,mouse cerebellum tissue,mouse NIH/3T3,rat PC-12 cell line,rat brain,rat cerebellum tissue,U-251 MG cell line (from left to right), using PITPNA Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from Hela cell line,mouse brain,mouse cerebellum tissue,mouse NIH/3T3,rat PC-12 cell line,rat brain,rat cerebellum tissue,U-251 MG cell line (from left to right), using PITPNA Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

Immunohistochemistry (IHC)-Paraffin

(Immunohistochemical analysis of paraffin-embedded H. skin section using PITPNA Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Immunohistochemistry (IHC)-Paraffin (Immunohistochemical analysis of paraffin-embedded H. skin section using PITPNA Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-PITPNA antibody
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Background: Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.
References
Dickeson S.K., et al. Gene 142:301-305(1994). Tanaka S., et al. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases. Lubec G., et al. Submitted (MAR-2007) to UniProtKB. Rikova K., et al. Cell 131:1190-1203(2007). Choudhary C., et al. Science 325:834-840(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31806
NCBI Official Full Name
phosphatidylinositol transfer protein alpha isoform
NCBI Official Synonym Full Names
phosphatidylinositol transfer protein alpha
NCBI Official Symbol
PITPNA
NCBI Official Synonym Symbols
PITPN; VIB1A; HEL-S-36; PI-TPalpha
NCBI Protein Information
phosphatidylinositol transfer protein alpha isoform
UniProt Protein Name
Phosphatidylinositol transfer protein alpha isoform
UniProt Gene Name
PITPNA
UniProt Synonym Gene Names
PITPN; PI-TP-alpha; PtdIns transfer protein alpha; PtdInsTP alpha
UniProt Entry Name
PIPNA_HUMAN

NCBI Description

This gene encodes a member of a family of lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces. The protein is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) by phosphoinositide-3-kinase.[provided by RefSeq, Sep 2009]

Uniprot Description

PITPNA: Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes. Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm

Molecular Function: phosphatidylinositol transporter activity; phosphatidylcholine transmembrane transporter activity; lipid binding

Biological Process: axon guidance; visual perception; phospholipid transport; lipid metabolic process

Research Articles on PITPNA

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Product Notes

The PITPNA pitpna (Catalog #AAA9231685) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PITPNA Antibody (C-term) reacts with Human, Mouse, Rat Predicted: Bovine, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's PITPNA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:1000 IHC-P: 1:25. Researchers should empirically determine the suitability of the PITPNA pitpna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PITPNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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