Canine Regulator Of G Protein Signaling 4 ELISA Kit | RGS4 elisa kit

Canine Regulator Of G Protein Signaling 4 ELISA Kit

Cat. Num. AAA739519

• Reactivity: Canine
• Synonyms: Regulator Of G Protein Signaling 4; Canine Regulator Of G Protein Signaling 4 ELISA Kit; RGS4 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This RGS4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine RGS4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGS4 elisa kit

Principle of the Assay: RGS4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RGS4 antibody and an RGS4-HRP conjugate. The assay sample and buffer are incubated together with RGS4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RGS4 concentration since RGS4 from samples and RGS4-HRP conjugate compete for the anti-RGS4 antibody binding site. Since the number of sites is limited, as more sites are occupied by RGS4 from the sample, fewer sites are left to bind RGS4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RGS4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RGS4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 55664920
• UniProt Accession #: P49798; A7XA56; A7XA58; A7XA59; A7YVV7; B1APZ3
• Molecular Weight: 21,452 Da
• UniProt Protein Name: Regulator of G-protein signaling 4
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS4
• UniProt Synonym Gene Names: RGP4; RGS4
• UniProt Entry Name: RGS4_HUMAN

Uniprot Description

RGS4: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, RGS

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: protein complex; cytoplasm; plasma membrane; cytosol

Molecular Function: calmodulin binding; G-protein alpha-subunit binding; GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; inactivation of MAPK activity; positive regulation of GTPase activity

Disease: Schizophrenia 9