Rabbit anti-Human TFR2 Polyclonal Antibody | anti-TFR2 antibody

TFR2, CT (TFR2, Transferrin receptor protein 2) (APC)

Cat. Num. AAA6355141

• Gene Names: TFR2; HFE3; TFRC2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TFR2; Polyclonal Antibody; CT (TFR2; Transferrin receptor protein 2) (APC); Transferrin receptor protein 2; anti-TFR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TFR2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TFR2 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 674-703 in the C-terminal region of human TFR2, UniProt Accession #NP_003218.2; Q9UP52.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292, K562 cell line lysates (35ug/lane) usingMBS6005924. This demonstrates that MBS6005924 detected the TFR2 protein (arrow).)

Related Product Information for anti-TFR2 antibody

This gene is a member of the transferrin receptor-like family and encodes a single-pass type II membrane protein with a protease associated (PA) domain, an M28 peptidase domain and a transferrin receptor-like dimerization domain. This protein mediates cellular uptake of transferrin-bound iron and mutations in this gene have been associated with hereditary hemochromatosis type III. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Product Categories/Family for anti-TFR2 antibody

Antibodies; Receptors

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). 3. Ikuta, K., et al. J. Mol. Biol. 397(2):375-384(2010). 4. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 5. Volke, M., et al. PLoS ONE 4 (11), E7875 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 33589848
• NCBI GeneID: 7036
• NCBI Accession #: NP_003218.2
• NCBI GenBank Nucleotide #: NM_003227.3
• UniProt Accession #: Q9UP52; O75422; Q1HE13; Q9HA99; Q9NX67; A6NGM7
• Molecular Weight: 88,755 Da
• NCBI Official Full Name: transferrin receptor protein 2 isoform 1
• NCBI Official Synonym Full Names: transferrin receptor 2
• NCBI Official Symbol: TFR2
• NCBI Official Synonym Symbols: HFE3; TFRC2
• NCBI Protein Information: transferrin receptor protein 2
• UniProt Protein Name: Transferrin receptor protein 2
• Protein Family: Transferrin receptor protein
• UniProt Gene Name: TFR2
• UniProt Synonym Gene Names: TfR2
• UniProt Entry Name: TFR2_HUMAN

Uniprot Description

TFR2: Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Defects in TFR2 are a cause of hemochromatosis type 3 (HFE3). HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects. Belongs to the peptidase M28 family. M28B subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: integral to plasma membrane; cytoplasm

Molecular Function: transferrin receptor activity

Biological Process: receptor-mediated endocytosis; cellular iron ion homeostasis; iron ion transport

Disease: Hemochromatosis, Type 3