Western Blot (WB)
(Western blot analysis of anti-PIP5K1B Pab in human placenta cell lysate. PIP5K1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human PIP5K1B Polyclonal Antibody | anti-PIP5K1B antibody
PIP5K1B Antibody (N-term)
Gene Names
PIP5K1B; MSS4; STM7
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PIP5K1B; Polyclonal Antibody; PIP5K1B Antibody (N-term); Phosphatidylinositol 4-phosphate 5-kinase type-1 beta; PIP5K1-beta; PtdIns(4)P-5-kinase 1 beta; Phosphatidylinositol 4-phosphate 5-kinase type I beta; PIP5KIbeta; Protein STM-7; Type I phosphatidylinositol 4-phosphate 5-kinase beta; STM7; anti-PIP5K1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PIP5K1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PIP5K1B.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1-30
Sequence Length
502
Applicable Applications for anti-PIP5K1B antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of anti-PIP5K1B Pab in human placenta cell lysate. PIP5K1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-PIP5K1B Pab in human placenta cell lysate. PIP5K1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PIP5K1B antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PIP5K1B antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-PIP5K1B antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Product Categories/Family for anti-PIP5K1B antibody
References
Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61036
NCBI Official Full Name
phosphatidylinositol 4-phosphate 5-kinase type-1 beta isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 5-kinase, type I, beta
NCBI Official Symbol
PIP5K1B
NCBI Official Synonym Symbols
MSS4; STM7
NCBI Protein Information
phosphatidylinositol 4-phosphate 5-kinase type-1 beta
UniProt Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 beta
Protein Family
UniProt Gene Name
PIP5K1B
UniProt Synonym Gene Names
STM7; PIP5K1-beta; PtdIns(4)P-5-kinase 1 beta; PIP5KIbeta
UniProt Entry Name
PI51B_HUMAN
Uniprot Description
PIP5K1B: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, lipid; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.68
Chromosomal Location of Human Ortholog: 9q13
Cellular Component: membrane; endomembrane system; uropod; cytosol
Molecular Function: protein binding; 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding
Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process
Research Articles on PIP5K1B
Similar Products
Product Notes
The PIP5K1B pip5k1b (Catalog #AAA9209903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The PIP5K1B Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIP5K1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the PIP5K1B pip5k1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIP5K1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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