Rabbit anti-Human PIP5K1A Polyclonal Antibody | anti-PIP5K1A antibody

PIP5K1A, NT (Phosphatidylinositol-4-phosphate 5-kinase Type-1 alpha, PtdIns(4)P-5-Kinase alpha, PIP5KI-alpha, Phosphatidylinositol-4-phosphate 5-kinase Type I alpha, 68kD Type I Phosphatidylinositol-4-phosphate 5-kinase alpha, PIP5KIalpha)

Cat. Num. AAA626587

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PIP5K1A; Polyclonal Antibody; NT (Phosphatidylinositol-4-phosphate 5-kinase Type-1 alpha; PtdIns(4)P-5-Kinase alpha; PIP5KI-alpha; Phosphatidylinositol-4-phosphate 5-kinase Type I alpha; 68kD Type I Phosphatidylinositol-4-phosphate 5-kinase alpha; PIP5KIalpha); Anti -PIP5K1A; anti-PIP5K1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIP5K1A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PIP5K1A antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human PIP5K1A (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PIP5K1A (arrow) using rabbit polyclonal hPIP5K1A-R34 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIP5K1A gene.)

Western Blot (WB)

(Western blot analysis of anti-PIP5K1A Pab in HeLa cell lysate. PIP5K1A (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-PIP5K1A antibody

Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.

Product Categories/Family for anti-PIP5K1A antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 208431778
• NCBI GeneID: 8394
• NCBI Accession #: NP_001129110.1
• NCBI GenBank Nucleotide #: NM_001135638.1
• UniProt Accession #: Q99755; Q99754; Q99756; A8K4Q0; P78517
• Molecular Weight: 62,633 Da
• NCBI Official Full Name: phosphatidylinositol-4-phosphate 5-kinase type-1 alpha isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
• NCBI Official Symbol: PIP5K1A
• NCBI Protein Information: phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; PIP5KIalpha; PIP5K1-alpha; OTTHUMP00000014289; OTTHUMP00000014290; OTTHUMP00000014291; OTTHUMP00000196331; OTTHUMP00000235065; ptdIns(4)P-5-kinase 1 alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha
• UniProt Protein Name: Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha
• Protein Family: Phosphatidylinositol 4-phosphate 5-kinase
• UniProt Gene Name: PIP5K1A
• UniProt Entry Name: PI51A_HUMAN

Uniprot Description

PIP5K1A: Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Interacts with RAC1. Interacts with TUT1. Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation. Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; Motility/polarity/chemotaxis; EC 2.7.1.68

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; focal adhesion; lamellipodium; cytoplasm; plasma membrane; nuclear speck; mRNA cleavage and polyadenylation specificity factor complex; nucleus; cytosol

Molecular Function: protein binding; 1-phosphatidylinositol-4-phosphate 5-kinase activity; kinase binding; ATP binding

Biological Process: focal adhesion formation; keratinocyte differentiation; cell migration; phosphoinositide phosphorylation; phospholipid metabolic process; actin cytoskeleton reorganization; phosphatidylinositol biosynthetic process; glycerophospholipid metabolic process; signal transduction; phagocytosis; phospholipid biosynthetic process