Western Blot (WB)
(Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit PION Polyclonal Antibody | anti-PION antibody
PION Antibody
MBS154323- Human Brain Tissue Slide.
Western Blot (WB)
(Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 μg/mL.Red: PION Antibody (6161)Blue: DAPI staining)
Immunofluorescence (IF)
(Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 μg/mL.Red: PION Antibody (6161)Blue: DAPI staining)
NCBI and Uniprot Product Information
NCBI Description
Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311), a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al., 2010 [PubMed 20811458]).[supplied by OMIM, Nov 2010]
Uniprot Description
GSAP: Regulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta- amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD. Belongs to the GSAP family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Activator
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: trans-Golgi network
Molecular Function: beta-amyloid binding
Biological Process: regulation of proteolysis
Research Articles on PION
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Product Notes
The PION gsap (Catalog #AAA151476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 770-820. The PION Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PION can be used in a range of immunoassay formats including, but not limited to, ELISA, IF, IHC-P, WB. PION antibody can be used for detection of PION by Western blot at 0.25 mg/mL. Antibody can also be used for immunohistochemistry starting at 5 mg/mL. For immunofluorescence start at 20 ug/mL. Researchers should empirically determine the suitability of the PION gsap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PION, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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