Rabbit anti-Human SLC25A13 Polyclonal Antibody | anti-SLC25A13 antibody

SLC25A13 Polyclonal Antibody

Cat. Num. AAA9132450

• Gene Names: SLC25A13; CTLN2; CITRIN; ARALAR2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: SLC25A13; Polyclonal Antibody; SLC25A13 Polyclonal Antibody; ARALAR2; CITRIN; CTLN2; anti-SLC25A13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 675

• Applicable Applications for anti-SLC25A13 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Immunogen: Recombinant protein of human SLC25A13
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SLC25A13 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using SLC25A13 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using SLC25A13 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-SLC25A13 antibody

This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-SLC25A13 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 13124095
• NCBI GeneID: 10165
• UniProt Accession #: Q9UJS0; O14566; O14575; Q546F9; Q9NZW1; Q9UNI7
• Molecular Weight: 74kDa
• NCBI Official Full Name: Calcium-binding mitochondrial carrier protein Aralar2
• NCBI Official Synonym Full Names: solute carrier family 25 member 13
• NCBI Official Symbol: SLC25A13
• NCBI Official Synonym Symbols: CTLN2; CITRIN; ARALAR2
• NCBI Protein Information: calcium-binding mitochondrial carrier protein Aralar2
• UniProt Protein Name: Calcium-binding mitochondrial carrier protein Aralar2
• Protein Family: Calcium-binding mitochondrial carrier protein
• UniProt Gene Name: SLC25A13
• UniProt Synonym Gene Names: ARALAR2

NCBI Description

This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

SLC25A13: Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2). Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years. Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD). NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms. Belongs to the mitochondrial carrier family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: acidic amino acid transmembrane transporter activity; calcium ion binding; L-aspartate transmembrane transporter activity; L-glutamate transmembrane transporter activity

Biological Process: aspartate transport; ATP biosynthetic process; cellular respiration; gluconeogenesis; L-glutamate transport; malate-aspartate shuttle; response to calcium ion

Disease: Citrullinemia, Type Ii, Adult-onset; Citrullinemia, Type Ii, Neonatal-onset