Western Blot (WB)
((0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PINX1 Polyclonal Antibody | anti-PINX1 antibody
Goat anti-PINX1 (aa225-237) Antibody
Gene Names
PINX1; LPTL; LPTS
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PINX1; Polyclonal Antibody; Goat anti-PINX1 (aa225-237) Antibody; Goat Anti-PINX1 Antibody; FLJ20565; LPTL; LPTS; MGC8850; 67-11-3 protein; hepatocellular carcinoma-related putative tumor suppressor; PIN2-interacting protein 1; Pin2-interacting protein X1; PINX1 antibody; FLJ20565 antibody; LPTL antibody; LPTS antibody; MGC8850 antibody; 67-11-3 protein antibody; hepatocellular carcinoma-related putative tumor suppressor antibody; PIN2-interacting protein 1 antibody; Pin2-interacting protein X1 antibody; PINX1 (aa225-237); anti-PINX1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ESYLQPKAKRHTE
Sequence Length
328
Applicable Applications for anti-PINX1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 40kDa band observed in lysates of cell lines A549, HepG2 and K562 (calculated MW of 37. 0kDa according to NP_060354.4). Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 40kDa band observed in lysates of cell lines A549, HepG2 and K562 (calculated MW of 37. 0kDa according to NP_060354.4). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-ESYLQPKAKRHTE, from the internal region of the protein sequence according to NP_060354.4.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,714 Da
NCBI Official Full Name
PIN2/TERF1-interacting telomerase inhibitor 1 isoform 1
NCBI Official Synonym Full Names
PIN2/TERF1 interacting, telomerase inhibitor 1
NCBI Official Symbol
PINX1
NCBI Official Synonym Symbols
LPTL; LPTS
NCBI Protein Information
PIN2/TERF1-interacting telomerase inhibitor 1
UniProt Protein Name
PIN2/TERF1-interacting telomerase inhibitor 1
Protein Family
UniProt Gene Name
PINX1
UniProt Synonym Gene Names
LPTL; LPTS
UniProt Entry Name
PINX1_HUMAN
Uniprot Description
PINX1: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. Belongs to the PINX1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleolus; Tumor suppressor; Inhibitor
Chromosomal Location of Human Ortholog: 8p23
Cellular Component: cytoplasm; intracellular membrane-bound organelle; kinetochore; mitochondrion; nuclear chromosome; nuclear chromosome, telomeric region; nucleolus; nucleus; spindle
Molecular Function: protein binding
Biological Process: mitotic metaphase plate congression; negative regulation of cell proliferation; negative regulation of protein ubiquitination; negative regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; regulation of protein stability; regulation of telomerase activity; telomere maintenance via telomerase
Research Articles on PINX1
1. PinX1 protein expression is decreased in colorectal cancer patients and predicts a poor prognosis.
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Product Notes
The PINX1 pinx1 (Catalog #AAA422201) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PINX1 (aa225-237) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PINX1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 40kDa band observed in lysates of cell lines A549, HepG2 and K562 (calculated MW of 37. 0kDa according to NP_060354.4). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the PINX1 pinx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ESYLQPKAKR HTE. It is sometimes possible for the material contained within the vial of "PINX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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