Rabbit anti-Human, Mouse PFDN1 Polyclonal Antibody | anti-PFDN1 antibody

PFDN1, CT (PFDN1, PFD1, Prefoldin subunit 1) (Azide free) (HRP)

Cat. Num. AAA6332841

• Gene Names: PFDN1; PDF; PFD1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PFDN1; Polyclonal Antibody; CT (PFDN1; PFD1; Prefoldin subunit 1) (Azide free) (HRP); Prefoldin subunit 1; anti-PFDN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PFDN1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PFDN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the C-terminal region of human PFDN1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PFDN1 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the PFDN1 antibody detected the PFDN1 protein (arrow).)

Related Product Information for anti-PFDN1 antibody

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils.

Product Categories/Family for anti-PFDN1 antibody

Antibodies; Proteins

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Stirling, P.C., et al. J. Biol. Chem. 281(11):7012-7021(2006). Stelzl, U., et al. Cell 122(6):957-968(2005). Simons, C.T., et al. J. Biol. Chem. 279(6):4196-4203(2004). Gstaiger, M., et al. Science 302(5648):1208-1212(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 21536449
• NCBI GeneID: 5201
• NCBI Accession #: NP_002613.2
• NCBI GenBank Nucleotide #: NM_002622.4
• UniProt Accession #: O60925; Q53F95; Q96EX6; B2RD02
• Molecular Weight: 14,210 Da
• NCBI Official Full Name: prefoldin subunit 1
• NCBI Official Synonym Full Names: prefoldin subunit 1
• NCBI Official Symbol: PFDN1
• NCBI Official Synonym Symbols: PDF; PFD1
• NCBI Protein Information: prefoldin subunit 1; prefoldin 1
• UniProt Protein Name: Prefoldin subunit 1
• Protein Family: Prefoldin
• UniProt Gene Name: PFDN1
• UniProt Synonym Gene Names: PFD1
• UniProt Entry Name: PFD1_HUMAN

NCBI Description

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008]

Uniprot Description

PFDN1: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: prefoldin complex

Molecular Function: unfolded protein binding; transcription factor activity

Biological Process: 'de novo' posttranslational protein folding; telencephalon development; cellular protein metabolic process; B cell activation; regulation of transcription, DNA-dependent; protein folding; cerebellum development; actin cytoskeleton organization and biogenesis; cell cycle