Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Pim-2 Oncogene (PIM2) Polyclonal Antibody | anti-PIM2 antibody
Polyclonal Antibody to Pim-2 Oncogene (PIM2)
Gene Names
Pim2; Pim-2; DXCch3
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Pim-2 Oncogene (PIM2); Polyclonal Antibody; Polyclonal Antibody to Pim-2 Oncogene (PIM2); anti-PIM2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PIM2. It has been selected for its ability to recognize PIM2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GKG GFGTVFAGHR VTDRRQVAIK VISRNRVLGW STVSDSVTCP LEVALLWKVG EGNGHPGVIR LLDWFETPEG FMLVLERPMP AQDLFDYITE KGPLGESCSR SFFTQVVAAV QHCHARGVVH RDIKDENILI DLCRGSIKLI DFGSGALLHD EPYTDFDGTR VYSPPEWISR HQYHALPATV WSLGVLLYDM VCGDIPFERD QEILEAELHF PAHVSPDCC
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GKG GFGTVFAGHR VTDRRQVAIK VISRNRVLGW STVSDSVTCP LEVALLWKVG EGNGHPGVIR LLDWFETPEG FMLVLERPMP AQDLFDYITE KGPLGESCSR SFFTQVVAAV QHCHARGVVH RDIKDENILI DLCRGSIKLI DFGSGALLHD EPYTDFDGTR VYSPPEWISR HQYHALPATV WSLGVLLYDM VCGDIPFERD QEILEAELHF PAHVSPDCC
Sequence Length
311
Applicable Applications for anti-PIM2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PIM2 (Gly98~Cys319) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2084614
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,487 Da
NCBI Official Full Name
serine/threonine-protein kinase pim-2
NCBI Official Synonym Full Names
proviral integration site 2
NCBI Official Symbol
Pim2
NCBI Official Synonym Symbols
Pim-2; DXCch3
NCBI Protein Information
serine/threonine-protein kinase pim-2
UniProt Protein Name
Serine/threonine-protein kinase pim-2
Protein Family
UniProt Gene Name
Pim2
UniProt Synonym Gene Names
Pim-2
Uniprot Description
Pim2: a Ca(2 )/calmodulin-dependent protein kinase of the PIM family. Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells.
Protein type: CAMK group; EC 2.7.11.1; Kinase, protein; Oncoprotein; PIM family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: X A1.1|X 3.55 cM
Cellular Component: cytoplasm
Molecular Function: ATP binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process: apoptosis; apoptotic mitochondrial changes; cell cycle; G1/S transition of mitotic cell cycle; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of protein binding; phosphorylation; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of macroautophagy; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; protein autophosphorylation; protein stabilization; regulation of mitotic cell cycle
Research Articles on PIM2
Similar Products
Product Notes
The PIM2 pim2 (Catalog #AAA2002127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Pim-2 Oncogene (PIM2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pim-2 Oncogene (PIM2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PIM2 pim2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GKG GFGTVFAGHR VTDRRQVAIK VISRNRVLGW STVSDSVTCP LEVALLWKVG EGNGHPGVIR LLDWFETPEG FMLVLERPMP AQDLFDYITE KGPLGESCSR SFFTQVVAAV QHCHARGVVH RDIKDENILI DLCRGSIKLI DFGSGALLHD EPYTDFDGTR VYSPPEWISR HQYHALPATV WSLGVLLYDM VCGDIPFERD QEILEAELHF PAHVSPDCC. It is sometimes possible for the material contained within the vial of "Pim-2 Oncogene (PIM2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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