Human cofilin 1 (non-muscle) ELISA Kit | CFL1 elisa kit

Human Cofilin-1, CFL1 ELISA Kit

Cat. Num. AAA929089

• Gene Names: CFL1; CFL; HEL-S-15
• Reactivity: Human
• Synonyms: cofilin 1 (non-muscle); Human Cofilin-1; CFL1 ELISA Kit; Human Cofilin-1 (CFL1) ELISA kit; CFL; ; CFL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CFL1. No significant cross-reactivity or interference between human CFL1 and analogues was observed.
• Sequence Length: 166

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 25 pg/ml-1600 pg/ml.
• Sensitivity: The minimum detectable dose of human CFL1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFL1 elisa kit

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CFL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CFL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CFL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CFL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5031635
• NCBI GeneID: 1072
• NCBI Accession #: NP_005498.1
• NCBI GenBank Nucleotide #: NM_005507.2
• UniProt Accession #: P23528; Q53Y87; Q9UCA2; B3KUQ1
• Molecular Weight: 18,502 Da
• NCBI Official Full Name: cofilin-1
• NCBI Official Synonym Full Names: cofilin 1 (non-muscle)
• NCBI Official Symbol: CFL1
• NCBI Official Synonym Symbols: CFL; HEL-S-15
• NCBI Protein Information: cofilin-1; p18; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: CFL1
• UniProt Synonym Gene Names: CFL; p18
• UniProt Entry Name: COF1_HUMAN

NCBI Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Uniprot Description

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Nuclear receptor co-regulator; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle

Molecular Function: protein binding; actin binding

Biological Process: axon guidance; platelet activation; actin filament depolymerization; positive regulation of actin filament depolymerization; response to virus; negative regulation of cell size; cytoskeleton organization and biogenesis; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; protein import into nucleus; establishment of cell polarity; ephrin receptor signaling pathway; regulation of cell morphogenesis; innate immune response; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis