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Western Blot (WB) (Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.)

Rabbit anti-Human PIK4CB Polyclonal Antibody | anti-PIK4CB antibody

PIK4CB (Phosphatidylinositol 4-kinase Catalytic beta, Phosphatidylinositol 4-kinase beta, PI4Kbeta, PI4K-beta, PI4KB, PI4KIIIbeta, PtdIns 4-kinase beta, PI4K92, NPIK) (AP)

Gene Names
PI4KB; NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
PIK4CB; Polyclonal Antibody; PIK4CB (Phosphatidylinositol 4-kinase Catalytic beta; Phosphatidylinositol 4-kinase beta; PI4Kbeta; PI4K-beta; PI4KB; PI4KIIIbeta; PtdIns 4-kinase beta; PI4K92; NPIK) (AP); EC=2.7.1.67; anti-PIK4CB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PIK4CB.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-PIK4CB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human PIK4CB (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PIK4CB antibody
References
1. Jeganathan, S., et.al., Mol. Cell. Biol. 28(14), 4549-4561 (2008). 2. Pizarro-Cerda, J., et.al., Cell. Microbiol. 9(10), 2381-2390 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
93kDa
NCBI Official Full Name
phosphatidylinositol 4-kinase beta isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase beta
NCBI Official Symbol
PI4KB
NCBI Official Synonym Symbols
NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
NCBI Protein Information
phosphatidylinositol 4-kinase beta
UniProt Protein Name
Phosphatidylinositol 4-kinase beta
UniProt Gene Name
PI4KB
UniProt Synonym Gene Names
PIK4CB; PI4K-beta; PI4Kbeta
UniProt Entry Name
PI4KB_HUMAN

Uniprot Description

PIK4CB: a member of the PI3/PI4 kinase family. Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex. May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Three splice-variant isoforms have been described.

Protein type: Kinase, lipid; EC 2.7.1.67; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: Golgi membrane; mitochondrial outer membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; rough endoplasmic reticulum membrane; cytosol; endosome

Molecular Function: 1-phosphatidylinositol 4-kinase activity; protein binding; ATP binding

Biological Process: receptor-mediated endocytosis; phosphoinositide-mediated signaling; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; signal transduction

Research Articles on PIK4CB

Similar Products

Product Notes

The PIK4CB pi4kb (Catalog #AAA6493508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIK4CB (Phosphatidylinositol 4-kinase Catalytic beta, Phosphatidylinositol 4-kinase beta, PI4Kbeta, PI4K-beta, PI4KB, PI4KIIIbeta, PtdIns 4-kinase beta, PI4K92, NPIK) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIK4CB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIK4CB pi4kb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK4CB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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