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Western Blot (WB) (Western Blot analysis of PIK3R5 expression in transfected 293T cell line by PIK3R5 polyclonal antibody. Lane 1: PIK3R5 transfected lysate (54.9kD). Lane 2: Non-transfected lysate.)

Rabbit anti-Human PIK3R5 Polyclonal Antibody | anti-PIK3R5 antibody

PIK3R5 (Phosphoinositide 3-kinase Regulatory Subunit 5, PI3-kinase p101 Subunit, Phosphatidylinositol 4,5-bisphosphate 3-kinase Regulatory Subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K) (AP)

Gene Names
PIK3R5; p101; FOAP-2; P101-PI3K; F730038I15Rik
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PIK3R5; Polyclonal Antibody; PIK3R5 (Phosphoinositide 3-kinase Regulatory Subunit 5; PI3-kinase p101 Subunit; Phosphatidylinositol 4; 5-bisphosphate 3-kinase Regulatory Subunit; Protein FOAP-2; PtdIns-3-kinase p101; p101-PI3K) (AP); anti-PIK3R5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PIK3R5.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
4359
Applicable Applications for anti-PIK3R5 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human PIK3R5, aa1-494 (AAH28212.1).
Immunogen Sequence
MDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PIK3R5 expression in transfected 293T cell line by PIK3R5 polyclonal antibody. Lane 1: PIK3R5 transfected lysate (54.9kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of PIK3R5 expression in transfected 293T cell line by PIK3R5 polyclonal antibody. Lane 1: PIK3R5 transfected lysate (54.9kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R5 and PIK3CG HeLa cells were stained with PIK3R5 rabbit purified polyclonal 1:1200 and PIK3CG mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Testing Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R5 and PIK3CG HeLa cells were stained with PIK3R5 rabbit purified polyclonal 1:1200 and PIK3CG mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Related Product Information for anti-PIK3R5 antibody
Receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit (Brock et al., 2003 [PubMed 12507995]).
Product Categories/Family for anti-PIK3R5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens phosphoinositide-3-kinase, regulatory subunit 5, mRNA
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 5
NCBI Official Symbol
PIK3R5
NCBI Official Synonym Symbols
p101; FOAP-2; P101-PI3K; F730038I15Rik
NCBI Protein Information
phosphoinositide 3-kinase regulatory subunit 5

NCBI Description

Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011]

Research Articles on PIK3R5

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Product Notes

The PIK3R5 (Catalog #AAA6389438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIK3R5 (Phosphoinositide 3-kinase Regulatory Subunit 5, PI3-kinase p101 Subunit, Phosphatidylinositol 4,5-bisphosphate 3-kinase Regulatory Subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3R5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIK3R5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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