Rabbit anti-Human PIK3R5 Polyclonal Antibody | anti-PIK3R5 antibody

PIK3R5 (Phosphoinositide 3-kinase Regulatory Subunit 5, PI3-kinase p101 Subunit, Phosphatidylinositol 4,5-bisphosphate 3-kinase Regulatory Subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K) (HRP)

Cat. Num. AAA6389442

• Gene Names: PIK3R5; p101; FOAP-2; P101-PI3K; F730038I15Rik
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIK3R5; Polyclonal Antibody; PIK3R5 (Phosphoinositide 3-kinase Regulatory Subunit 5; PI3-kinase p101 Subunit; Phosphatidylinositol 4; 5-bisphosphate 3-kinase Regulatory Subunit; Protein FOAP-2; PtdIns-3-kinase p101; p101-PI3K) (HRP); anti-PIK3R5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIK3R5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 4359

• Applicable Applications for anti-PIK3R5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PIK3R5, aa1-494 (AAH28212.1).
• Immunogen Sequence: MDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PIK3R5 expression in transfected 293T cell line by PIK3R5 polyclonal antibody. Lane 1: PIK3R5 transfected lysate (54.9kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R5 and PIK3CG HeLa cells were stained with PIK3R5 rabbit purified polyclonal 1:1200 and PIK3CG mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Related Product Information for anti-PIK3R5 antibody

Receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit (Brock et al., 2003 [PubMed 12507995]).

Product Categories/Family for anti-PIK3R5 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 24081055
• NCBI GeneID: 23533
• NCBI Official Full Name: Homo sapiens phosphoinositide-3-kinase, regulatory subunit 5, mRNA
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 5
• NCBI Official Symbol: PIK3R5
• NCBI Official Synonym Symbols: p101; FOAP-2; P101-PI3K; F730038I15Rik
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 5
• Protein Family: Phosphoinositide 3-kinase regulatory

NCBI Description

Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011]