Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.)
Rabbit anti-Human; Rat PIK3R4 Polyclonal Antibody | anti-PIK3R4 antibody
PIK3R4 Antibody: Alkaline Phosphatase
Gene Names
PIK3R4; p150; VPS15
Reactivity
Human; Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Peptide Affinity Purified
Synonyms
PIK3R4; Polyclonal Antibody; PIK3R4 Antibody: Alkaline Phosphatase; Rabbit Anti-Human PIK3R4 Polyclonal; VPS15 Antibody; Phosphoinositide 3-kinase regulatory subunit 4 Antibody; PI3-kinase regulatory subunit 4 Antibody; PI3-kinase p150 subunit Antibody; PIK3R4 Antibody; Phosphoinositide 3-kinase adaptor protein Antibody; anti-PIK3R4 antibody
Host
Rabbit
Reactivity
Human; Rat
Clonality
Polyclonal
Specificity
Detects ~160 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
1358
Applicable Applications for anti-PIK3R4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000
ICC/IF: 1:100
ICC/IF: 1:100
Target
PIK3R4
Immunogen
Synthetic peptide from the C-terminal of Human PIK3R4 (aa. 1314-1325)
Immunogen Species
Human
Cellular Localization
Late Endosome; Cytoplasmic Vesicle; Autophagosome; Membrane; Lipid-Anchor
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitously expressed.
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.)
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.)
Product Categories/Family for anti-PIK3R4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
153,103 Da
NCBI Official Full Name
phosphoinositide 3-kinase regulatory subunit 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 4
NCBI Official Symbol
PIK3R4
NCBI Official Synonym Symbols
p150; VPS15
NCBI Protein Information
phosphoinositide 3-kinase regulatory subunit 4
UniProt Protein Name
Phosphoinositide 3-kinase regulatory subunit 4
Protein Family
UniProt Gene Name
PIK3R4
UniProt Synonym Gene Names
; PI3-kinase regulatory subunit 4
Uniprot Description
Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).
Research Articles on PIK3R4
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Product Notes
The PIK3R4 pik3r4 (Catalog #AAA809859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIK3R4 Antibody: Alkaline Phosphatase reacts with Human; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3R4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ICC/IF: 1:100. Researchers should empirically determine the suitability of the PIK3R4 pik3r4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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