Porcine Alpha-Fetoprotein ELISA Kit | alphaFP elisa kit

Porcine Alpha-Fetoprotein ELISA Kit

Cat. Num. AAA760999

• Gene Names: AFP; AFPD; FETA; HPAFP
• Reactivity: Porcine
• Synonyms: Alpha-Fetoprotein; Porcine Alpha-Fetoprotein ELISA Kit; alphaFP elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of alpha FP. No significant cross-reactivity or interference betweenalpha FP and analogues was observed.
• Sequence Length: 609

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level alpha FP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level alpha FP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for alphaFP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- alpha FP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-alpha FP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the alphaFP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of alpha FP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4501989
• NCBI GeneID: 174
• NCBI Accession #: NP_001125.1
• NCBI GenBank Nucleotide #: NM_001134.2
• Molecular Weight: 68,678 Da
• NCBI Official Full Name: alpha-fetoprotein
• NCBI Official Synonym Full Names: alpha fetoprotein
• NCBI Official Symbol: AFP
• NCBI Official Synonym Symbols: AFPD; FETA; HPAFP
• NCBI Protein Information: alpha-fetoprotein
• UniProt Protein Name: Alpha-fetoprotein
• Protein Family: Alpha-fetoprotein
• UniProt Gene Name: AFP
• UniProt Synonym Gene Names: HPAFP
• UniProt Entry Name: FETA_HUMAN

NCBI Description

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Uniprot Description

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytoplasm; extracellular space

Molecular Function: metal ion binding

Biological Process: liver development; organ regeneration; ovulation from ovarian follicle; pancreas development; progesterone metabolic process; response to organic substance; transport

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of