Rabbit anti-Human PIK3R1 Polyclonal Antibody | anti-PIK3R1 antibody

PIK3R1 (GRB1, Phosphatidylinositol 3-kinase Regulatory Subunit alpha, Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (AP)

Cat. Num. AAA6389427

• Gene Names: PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIK3R1; Polyclonal Antibody; PIK3R1 (GRB1; Phosphatidylinositol 3-kinase Regulatory Subunit alpha; Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (AP); anti-PIK3R1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIK3R1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 5712

• Applicable Applications for anti-PIK3R1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PIK3R1, aa1-424.
• Immunogen Sequence: MHNLQTLPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 polyclonal antibody. Lane 1: PIK3R1 transfected lysate (50kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between PIK3R1 and HRAS HeLa cells were stained with PIK3R1 rabbit purified polyclonal 1:1200 and HRAS mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between PIK3R1 and SHC1 Huh7 cells were stained with PIK3R1 rabbit purified polyclonal 1:1200 and SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Related Product Information for anti-PIK3R1 antibody

PI3KR1 binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the P110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. It is a component of a heterodimer composed of P110 (catalytic) and P85 (regulatory) subunits. PI3KR1 is proposed to interact with phosphorylated TOM1L1. Defects in PIK3R1 are a cause of severe insulin resistance. The protein contains 1 Rho-GAP domain, 2 SH2 domains, and 1 SH3 domain.

Product Categories/Family for anti-PIK3R1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 1676319999
• NCBI GeneID: 5295
• NCBI Accession #: NM_181524.2
• NCBI GenBank Nucleotide #: NM_181524
• NCBI Official Full Name: Homo sapiens phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1), transcript variant 3, mRNA
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 1
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; IMD36; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory

NCBI Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]