Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using HNF4a antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human, Mouse HNF4a Polyclonal Antibody | anti-HNF4a antibody
HNF4a Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using HNF4a antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using HNF4a antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcription factor involved in the development of the pancreas, liver, kidney, and intestines. The encoded protein also functions to maintain glucose homeostasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
Uniprot Description
HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.
Protein type: DNA-binding; Nuclear receptor; Transcription factor
Chromosomal Location of Human Ortholog: 2 H3|2 84.32 cM
Cellular Component: cytoplasm; nucleoplasm; nucleus; transcription factor complex
Molecular Function: acyl-CoA binding; DNA binding; fatty acid binding; palmitoyl-CoA hydrolase activity; protein binding; protein homodimerization activity; receptor binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: acyl-CoA metabolic process; blood coagulation; cell differentiation; establishment of tissue polarity; glucose homeostasis; intercellular junction assembly and maintenance; lipid homeostasis; lipid metabolic process; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of mitotic cell cycle; negative regulation of protein import into nucleus, translocation; negative regulation of transcription factor activity; negative regulation of tyrosine phosphorylation of STAT protein; ornithine metabolic process; phospholipid homeostasis; positive regulation of fatty acid biosynthetic process; positive regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of gastrulation; regulation of insulin secretion; regulation of lipid metabolic process; regulation of microvillus biogenesis; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to glucose stimulus; sex differentiation; xenobiotic metabolic process
Research Articles on HNF4a
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Product Notes
The HNF4a hnf4a (Catalog #AAA9131535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HNF4a Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HNF4a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the HNF4a hnf4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF4a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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