Western Blot (WB)
(Western blot analysis of Jurkat and Hela cell lines, using PIK3CG antibody.)
Rabbit PIK3CG Polyclonal Antibody | anti-PIK3CG antibody
PIK3CG (Phosphoinositide 3 Kinase, catalytic, Phosphoinositide-3-kinase, Catalytic, Gamma Polypeptide, PI3K, PIK3, PI3CG, PI3Kgamma) (AP)
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of Jurkat and Hela cell lines, using PIK3CG antibody.)
Western Blot (WB)
(Western blot analysis of Jurkat and Hela cell lines, using PIK3CG antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. [provided by RefSeq, Jul 2008]
Uniprot Description
PIK3CG: catalytic subunit of the PI3/PI4-kinase family of phospholipid-kinases. Phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. Phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 recruits proteins with PH domains to the membrane, including AKT1 and PDPK1, and plays a critical pleiotropic role in regulating membrane signaling. Activated by both the alpha and the beta:gamma G proteins following stimulation of G protein-coupled receptors (GPCRs), linking GPCR activation to PIP3 production. Activation by GPCRs is assisted by the regulatory subunits (PIK3R5 or PIK3R6), leading to the translocation from the cytosol to the plasma membrane. Inhibited by AS-604850 and AS-605240. Activates signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD, it regulates many cellular functions: it is involved in natural killer (NK) cell development and migration towards the sites of inflammation; participates in T-lymphocyte development and migration; required for B-lymphocyte development and signaling; regulates lymphocyte cytokine production; participates in neutrophil respiratory burst, chemotaxis and extravasation; promotes platelet aggregation and thrombosis; regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism; involved in endothelial progenitor cell migration; modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels; regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to ADRBK1 and by non-muscle tropomyosin phosphorylation; and contributes to cardiac hypertrophy under pathological stress. Possesses serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. Holoenzyme is a trimer composed of a heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with HRAS1; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6.
Protein type: Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 2.7.1.153; EC 2.7.11.1; Kinase, lipid; Autophagy
Chromosomal Location of Human Ortholog: 7q22.3
Cellular Component: mast cell granule; membrane; cytoplasm; plasma membrane; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; ephrin receptor binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; ATP binding; protein kinase activity
Biological Process: respiratory burst during defense response; platelet activation; neutrophil chemotaxis; phosphoinositide 3-kinase cascade; adaptive immune response; T cell activation; cytokine production; endocytosis; mast cell degranulation; protein amino acid phosphorylation; T cell proliferation; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; positive regulation of MAP kinase activity; regulation of cell adhesion mediated by integrin; elevation of cytosolic calcium ion concentration; positive regulation of acute inflammatory response; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; innate immune response; dendritic cell chemotaxis; angiogenesis; blood coagulation; inflammatory response; secretory granule localization
Research Articles on PIK3CG
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Product Notes
The PIK3CG pik3cg (Catalog #AAA6431611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIK3CG (Phosphoinositide 3 Kinase, catalytic, Phosphoinositide-3-kinase, Catalytic, Gamma Polypeptide, PI3K, PIK3, PI3CG, PI3Kgamma) (AP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3CG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIK3CG pik3cg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3CG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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