Mouse TRIM28 / KAP-1 Monoclonal Antibody | anti-TRIM28 antibody

Mouse Monoclonal [clone 4E6] (IgG2b,k) to Human TRIM28 / KAP-1

Cat. Num. AAA246598

• Gene Names: TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: TRIM28 / KAP-1; Monoclonal Antibody; Mouse Monoclonal [clone 4E6] (IgG2b; k) to Human TRIM28 / KAP-1; Anti-TRIM28 / KAP-1 Antibody (clone 4E6) IHC-plus; TRIM28; E3 SUMO-protein ligase TRIM28; KRIP-1; Nuclear corepressor KAP-1; KAP-1; KAP1; KRAB-interacting protein 1; TIF1B; RNF96; TIF1-beta; TIF1beta; Tripartite motif-containing 28; KRAB-associated protein 1; RING finger protein 96; TF1B; Human TRIM28; anti-TRIM28 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 4E6
• Specificity: Human TRIM28
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 835

• Applicable Applications for anti-TRIM28 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Western Blot: Cell lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: TRIM28 / KAP-1 antibody was raised against tRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TRIM28 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody, clone 4E6.)

Western Blot (WB)

(TRIM28 monoclonal antibody, clone 4E6. Western blot of TRIM28 expression in PC-12.)

RNA interference

(Western blot of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody clone 4E6. GAPDH (36.1 kD) used as control.)

Related Product Information for anti-TRIM28 antibody

TIF1beta is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily. TIF1beta contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TIF1beta is a corepressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TIF1beta acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors.

Product Categories/Family for anti-TRIM28 antibody

Family: Tripartite Motif; Subfamily: TIF1

NCBI and Uniprot Product Information

• NCBI GI #: 5032179
• NCBI GeneID: 10155
• NCBI Accession #: NP_005753.1
• NCBI GenBank Nucleotide #: NM_005762.2
• UniProt Accession #: Q13263; O00677; Q7Z632; Q93040; Q96IM1
• Molecular Weight: 79,474 Da
• NCBI Official Full Name: transcription intermediary factor 1-beta
• NCBI Official Synonym Full Names: tripartite motif containing 28
• NCBI Official Symbol: TRIM28
• NCBI Official Synonym Symbols: KAP1; TF1B; RNF96; TIF1B; PPP1R157
• NCBI Protein Information: transcription intermediary factor 1-beta; KAP-1; KRIP-1; TIF1-beta; RING finger protein 96; KRAB-associated protein 1; nuclear corepressor KAP-1; KRAB-interacting protein 1; E3 SUMO-protein ligase TRIM28; tripartite motif-containing 28; tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; protein phosphatase 1, regulatory subunit 157; KRAB [Kruppel-associated box domain]-associated protein 1
• UniProt Protein Name: Transcription intermediary factor 1-beta
• Protein Family: Transcription intermediary factor
• UniProt Gene Name: TRIM28
• UniProt Synonym Gene Names: KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
• UniProt Entry Name: TIF1B_HUMAN

NCBI Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Protein type: Ligase; Transcription, coactivator/corepressor; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Kinase, protein; Protein kinase, atypical; Nuclear receptor co-regulator; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription corepressor activity; transcription factor activity; protein kinase activity; ligase activity

Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; epithelial to mesenchymal transition; innate immune response; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation during embryonic development; embryo implantation