Rabbit anti-Human PIK3CG Polyclonal Antibody | anti-PIK3CG antibody

PIK3CG, Phosphorylated (Ser1100) (Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit gamma Isoform, PtdIns-3-kinase Subunit gamma, PI3K-gamma, Phosphatidylinositol-4,5-bisphosphate 3-kinase 110kD Catalytic Subunit gamma, PtdIns-3-kinase Subu

Cat. Num. AAA6493435

• Gene Names: PIK3CG; PI3K; PIK3; PI3CG; PI3Kgamma; p110gamma; p120-PI3K
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PIK3CG; Polyclonal Antibody; Phosphorylated (Ser1100) (Phosphatidylinositol-4; 5-bisphosphate 3-kinase Catalytic Subunit gamma Isoform; PtdIns-3-kinase Subunit gamma; PI3K-gamma; Phosphatidylinositol-4; 5-bisphosphate 3-kinase 110kD Catalytic Subunit gamma; PtdIns-3-kinase Subu; anti-PIK3CG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIK3CG when phosphorylated at Ser1100.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PIK3CG antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser1100 of human PIK3CG (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-PIK3CG-pS1100 Phospho-specific Pab (RB13320) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-PIK3CG antibody

Antibodies; Protein Kinases

References

1. Andreozzi, F., et al., Endocrinology 145(6):2845-2857 (2004). 2. Reddy, S.A., et al., Biochem. Biophys. Res. Commun. 316(4):1022-1028 (2004). 3. Osaki, M., et al., J. Cancer Res. Clin. Oncol. 130(1):8-14 (2004). 4. Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003). 5. Theberge, J.F., et al., Arch. Biochem. Biophys. 420(1):9-17 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 21237725
• NCBI GeneID: 5294
• NCBI Accession #: NP_002640
• NCBI GenBank Nucleotide #: NM_002649
• UniProt Accession #: P48736; Q8IV23; Q9BZC8; A4D0Q6
• Molecular Weight: 126,454 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma
• NCBI Official Symbol: PIK3CG
• NCBI Official Synonym Symbols: PI3K; PIK3; PI3CG; PI3Kgamma; p110gamma; p120-PI3K
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; 1-phosphatidylinositol 3-kinase; PI3-kinase subunit gamma; phosphatidylinositol 3 kinase gamma, p110 gamma; phosphatidylinositol 3-kinase catalytic 110-kD gamma; phosphatidyli
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: PIK3CG
• UniProt Synonym Gene Names: PI3-kinase subunit gamma; PI3K-gamma; PI3Kgamma; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma; p110gamma
• UniProt Entry Name: PK3CG_HUMAN

Uniprot Description

PIK3CG: catalytic subunit of the PI3/PI4-kinase family of phospholipid-kinases. Phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. Phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 recruits proteins with PH domains to the membrane, including AKT1 and PDPK1, and plays a critical pleiotropic role in regulating membrane signaling. Activated by both the alpha and the beta:gamma G proteins following stimulation of G protein-coupled receptors (GPCRs), linking GPCR activation to PIP3 production. Activation by GPCRs is assisted by the regulatory subunits (PIK3R5 or PIK3R6), leading to the translocation from the cytosol to the plasma membrane. Inhibited by AS-604850 and AS-605240. Activates signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD, it regulates many cellular functions: it is involved in natural killer (NK) cell development and migration towards the sites of inflammation; participates in T-lymphocyte development and migration; required for B-lymphocyte development and signaling; regulates lymphocyte cytokine production; participates in neutrophil respiratory burst, chemotaxis and extravasation; promotes platelet aggregation and thrombosis; regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism; involved in endothelial progenitor cell migration; modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels; regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to ADRBK1 and by non-muscle tropomyosin phosphorylation; and contributes to cardiac hypertrophy under pathological stress. Possesses serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. Holoenzyme is a trimer composed of a heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with HRAS1; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6.

Protein type: Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 2.7.1.153; EC 2.7.11.1; Kinase, lipid; Autophagy

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: mast cell granule; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; ephrin receptor binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; ATP binding; protein kinase activity

Biological Process: respiratory burst during defense response; platelet activation; neutrophil chemotaxis; phosphoinositide 3-kinase cascade; adaptive immune response; T cell activation; cytokine production; endocytosis; mast cell degranulation; protein amino acid phosphorylation; T cell proliferation; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; positive regulation of MAP kinase activity; regulation of cell adhesion mediated by integrin; elevation of cytosolic calcium ion concentration; positive regulation of acute inflammatory response; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; innate immune response; dendritic cell chemotaxis; angiogenesis; blood coagulation; inflammatory response; secretory granule localization