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Western Blot (WB) (Mouse Eif2ak4 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Eif2ak4 antibody detected the Eif2ak4 protein (arrow).)

Rabbit anti-Mouse Eif2ak4 Polyclonal Antibody | anti-Eif2ak4 antibody

Eif2ak4, CT (Eif2ak4, Gcn2, Kiaa1338, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein) (FITC)

Gene Names
Eif2ak4; GCN2; MGCN2; 2610011M03
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Eif2ak4; Polyclonal Antibody; CT (Eif2ak4; Gcn2; Kiaa1338; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein) (FITC); GCN2-like protein; anti-Eif2ak4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-Eif2ak4 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
Mouse Eif2ak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1476-1504 amino acids from the C-terminal region of Mouse Eif2ak4.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Eif2ak4 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Eif2ak4 antibody detected the Eif2ak4 protein (arrow).)

Western Blot (WB) (Mouse Eif2ak4 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Eif2ak4 antibody detected the Eif2ak4 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Eif2ak4 Antibody (C-term) (AP14713b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse live tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Eif2ak4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Mouse Eif2ak4 Antibody (C-term) (AP14713b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse live tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Eif2ak4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Eif2ak4 antibody
Eif2ak4 can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
Product Categories/Family for anti-Eif2ak4 antibody
References
Bunpo, P., et al. J. Nutr. 140(11):2020-2027(2010). Ye, J., et al. EMBO J. 29(12):2082-2096(2010). Liu, Y., et al. Neoplasia 12(1):61-68(2010). Jasperson, L.K., et al. Blood 114(24):5062-5070(2009). Bunpo, P., et al. J. Biol. Chem. 284(47):32742-32749(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
186,487 Da
NCBI Official Full Name
eukaryotic translation initiation factor 2-alpha kinase 4 isoform 2
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 alpha kinase 4
NCBI Official Symbol
Eif2ak4
NCBI Official Synonym Symbols
GCN2; MGCN2; 2610011M03
NCBI Protein Information
eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; GCN2 eIF2alpha kinase
UniProt Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 4
Protein Family
UniProt Gene Name
Eif2ak4
UniProt Synonym Gene Names
Gcn2; Kiaa1338; mGCN2
UniProt Entry Name
E2AK4_MOUSE

Uniprot Description

GCN2: a kinase of the PEK family. Phosphorylates and inhibits eukaryotic initiation factor (eIF)-2 alpha, providing an important protective mechanism during various cellular stress conditions including endoplasmic reticulum (ER) stress.

Protein type: Protein kinase, Other; Kinase, protein; Translation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; PEK family; GCN2 subfamily

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein homodimerization activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of translation initiation in response to stress; unfolded protein response; negative regulation of translation; cellular response to starvation; protein amino acid phosphorylation; phosphorylation; regulation of translational initiation

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Product Notes

The Eif2ak4 eif2ak4 (Catalog #AAA6294866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eif2ak4, CT (Eif2ak4, Gcn2, Kiaa1338, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein) (FITC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Eif2ak4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Eif2ak4 eif2ak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eif2ak4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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